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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EphA4 Polyclonal antibody (21875-1-AP)

    WB analysis using 21875-1-AP (same clone as 21875-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21875-1-AP (EphA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21875-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using EphA4 Polyclonal antibody (21875-1-AP)

    IP experiment of mouse brain using 21875-1-AP (same clone as 21875-1-PBS)

    IP result of anti-EphA4 (IP:21875-1-AP, 4ug; Detection:21875-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 21875-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EphA4 Polyclonal antibody (21875-1-AP)

    IHC staining of mouse brain using 21875-1-AP (same clone as 21875-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21875-1-AP (EphA4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21875-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EphA4 Polyclonal antibody (21875-1-AP)

    IHC staining of mouse brain using 21875-1-AP (same clone as 21875-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21875-1-AP (EphA4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21875-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Neuron cells using EphA4 Polyclonal antibody (21875-1-AP)

    IF Staining of Neuron cells using 21875-1-AP (same clone as 21875-1-PBS)

    IF result of EphA4 antibody (21875-1-AP, 1:50) with culture neuron cells by Wilson Pak Kin Lou. This data was developed using the same antibody clone with 21875-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

EphA4 Polyclonal antibody, PBS Only

EphA4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 21875-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21875-1-PBS targets EphA4 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EphA4 fusion protein Ag16233 相同性解析による交差性が予測される生物種
Full Name EPH receptor A4
Calculated molecular weight 986 aa, 110 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC026327
Gene Symbol EPHA4
Gene ID (NCBI) 2043
RRIDAB_2878936
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP54764
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

EphA4 is a member of the Eph receptor tyrosine kinase family and has important functions in the developing and adult nervous system (PMID: 14697664). The Eph receptors comprise a large family of closely related transmembrane tyrosine kinases that actively signal when bound to their ephrin ligands. The Eph receptors are characterized by an extracellular region with a unique cysteine-rich motif extending over its amino-terminal half, followed by two fibronectin type III motifs (PMID: 9530499). They are divided into two sub-groups (EphA and EphB) based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands (PMID: 11114742). EphA4 is involved in commissure formation within the forebrain, axonal guidance in the corticospinal tract, regulation of the central pattern generator that provides normal locomotor function and axonal regeneration following spinal cord injury (PMID: 30061574). EphA4 has been implicated as a disease modifier of amyotrophic lateral sclerosis (ALS) (PMID: 22922411).