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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Exportin 4 Recombinant antibody (84449-1-RR)

    WB analysis using 84449-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84449-1-RR (XPO4 antibody) at dilution of 1:8500 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using Exportin 4 Recombinant antibody (84449-1-RR)

    FC experiment of HeLa using 84449-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Exportin 4 Recombinant antibody (84449-1-RR, Clone:241443A5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84449-1-RR

    Affinity and Kinetic Characterization of 84449-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84449-1-RR against Human Exportin 4 were performed. The affinity constant is 0.512 nM.

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Exportin 4 Recombinant antibody

Exportin 4 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84449-1-RR
Clone No.241443A5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

XPO4, KIAA1721, Exportin-4, Exp4, 241443A5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, SW480 cells, RAW 264.7 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:17000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84449-1-RR targets Exportin 4 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Exportin 4 fusion protein Ag21845 相同性解析による交差性が予測される生物種
Full Name exportin 4
Calculated molecular weight 1151 aa, 130 kDa
Observed molecular weight130 kDa
GenBank accession numberBC113571
Gene Symbol Exportin 4/XPO4
Gene ID (NCBI) 64328
RRIDAB_3671975
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9C0E2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

exportin 4 (XPO4) maps to chromosome 13q and belongs to the importin β family which acts as a bidirectional nuclear transport receptor. Decreased XPO4 expression has been shown to associate with hepatocellular carcinoma. A genome-wide copy number variation (CNV) association study in a small cohort of Malaysian subjects identified a CNV in XPO4 to be correlated with the progression of metabolic (dysfunction) associated fatty liver disease (MAFLD). (PMID: 34388518)

Protocols

Product Specific Protocols
WB protocol for Exportin 4 antibody 84449-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols