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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

JAM-A/Junctional Adhesion Molecule A Polyclonal antibody

JAM-A/Junctional Adhesion Molecule A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26941-1-AP

Synonyms

F11R, JAM A, JAM 1, JAM, F11 receptor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using JAM-A/Junctional Adhesion Molecule A Polyclonal an (26941-1-AP)

    WB analysis using 26941-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26941-1-AP (JAM-A/Junctional Adhesion Molecule A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using JAM-A/Junctional Adhesion Molecule A Polyclonal an (26941-1-AP)

    WB analysis using 26941-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26941-1-AP (JAM-A/Junctional Adhesion Molecule A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human peripheral blood platelets using JAM-A/Junctional Adhesion Molecule A Polyclonal an (26941-1-AP)

    WB analysis of human peripheral blood platelets using 26941-1-AP

    Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 26941-1-AP (JAM-A/Junctional Adhesion Molecule A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using JAM-A/Junctional Adhesion Molecule A Polyclonal an (26941-1-AP)

    IHC staining of human breast cancer using 26941-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26941-1-AP (JAM-A/Junctional Adhesion Molecule A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using JAM-A/Junctional Adhesion Molecule A Polyclonal an (26941-1-AP)

    IHC staining of human breast cancer using 26941-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26941-1-AP (JAM-A/Junctional Adhesion Molecule A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using JAM-A/Junctional Adhesion Molecule A Polyclonal an (26941-1-AP)

    IHC staining of human spleen using 26941-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 26941-1-AP (JAM-A/Junctional Adhesion Molecule A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using JAM-A/Junctional Adhesion Molecule A Polyclonal an (26941-1-AP)

    IHC staining of human spleen using 26941-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 26941-1-AP (JAM-A/Junctional Adhesion Molecule A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHUVEC cells, human peripheral blood platelets, U-87 MG cells
Positive IHC detected inhuman breast cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26941-1-AP targets JAM-A/Junctional Adhesion Molecule A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen JAM-A/Junctional Adhesion Molecule A fusion protein Ag25641 相同性解析による交差性が予測される生物種
Full Name F11 receptor
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC001533
Gene symbol F11R
Gene ID (NCBI) 50848
RRIDAB_2880692
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The F11 receptor (F11R) was first identified in human platelets as a 32/35 kDa protein duplex that serves as the receptor for a functional antibody that activates platelets. It seems to play a role in epithelial tight junction formation. It was also reported to function as a receptor for reovirus and ligand for the integrin LFA1.

Protocols

Product Specific Protocols
WB protocol for JAM-A/Junctional Adhesion Molecule A antibody 26941-1-APDownload protocol
IHC protocol for JAM-A/Junctional Adhesion Molecule A antibody 26941-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols