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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

F11R Polyclonal antibody

F11R Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26941-1-AP

Synonyms

CD321, F11 receptor, F11R, JAM, JAM 1, JAM A, JAM1, JAMA, JAMA1, JCAM, Junctional adhesion molecule 1, Junctional adhesion molecule A, KAT, PAM 1, Platelet adhesion molecule 1, Platelet F11 receptor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using F11R Polyclonal antibody (26941-1-AP)

    WB analysis using 26941-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26941-1-AP (F11R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using F11R Polyclonal antibody (26941-1-AP)

    WB analysis using 26941-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26941-1-AP (F11R antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human peripheral blood platelets using F11R Polyclonal antibody (26941-1-AP)

    WB analysis of human peripheral blood platelets using 26941-1-AP

    Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 26941-1-AP (F11R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using F11R Polyclonal antibody (26941-1-AP)

    IHC staining of human breast cancer using 26941-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26941-1-AP (F11R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using F11R Polyclonal antibody (26941-1-AP)

    IHC staining of human breast cancer using 26941-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26941-1-AP (F11R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using F11R Polyclonal antibody (26941-1-AP)

    IHC staining of human spleen using 26941-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 26941-1-AP (F11R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using F11R Polyclonal antibody (26941-1-AP)

    IHC staining of human spleen using 26941-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 26941-1-AP (F11R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHUVEC cells, human peripheral blood platelets, U-87 MG cells
Positive IHC detected inhuman breast cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26941-1-AP targets F11R in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen F11R fusion protein Ag25641 相同性解析による交差性が予測される生物種
Full Name F11 receptor
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC001533
Gene symbol F11R
Gene ID (NCBI) 50848
RRIDAB_2880692
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The F11 receptor (F11R) was first identified in human platelets as a 32/35 kDa protein duplex that serves as the receptor for a functional antibody that activates platelets. It seems to play a role in epithelial tight junction formation. It was also reported to function as a receptor for reovirus and ligand for the integrin LFA1.

Protocols

Product Specific Protocols
WB protocol for F11R antibody 26941-1-APDownload protocol
IHC protocol for F11R antibody 26941-1-APDownload protocol
FC protocol for F11R antibody 26941-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols