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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Factor XII Polyclonal antibody

Factor XII Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27154-1-AP

Synonyms

Coagulation factor XII, F12, Factor XII, HAE3, HAEX, HAF, Hageman factor

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Factor XII Polyclonal antibody (27154-1-AP)

    WB analysis of human plasma using 27154-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 27154-1-AP (F12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using Factor XII Polyclonal antibody (27154-1-AP)

    WB analysis of K-562 using 27154-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 27154-1-AP (F12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Factor XII Polyclonal antibody (27154-1-AP)

    WB analysis of HepG2 using 27154-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27154-1-AP (F12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using Factor XII Polyclonal antibody (27154-1-AP)

    WB analysis of Jurkat using 27154-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 27154-1-AP (F12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using Factor XII Polyclonal antibody (27154-1-AP)

    IHC staining of human liver using 27154-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27154-1-AP (F12 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Factor XII Polyclonal antibody (27154-1-AP)

    IHC staining of human liver using 27154-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27154-1-AP (F12 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inhuman plasma tissue, K-562 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27154-1-AP is Factor XII Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Factor XII fusion protein Ag25912 相同性解析による交差性が予測される生物種
Full Name coagulation factor XII (Hageman factor)
Calculated molecular weight 615 aa, 68 kDa
Observed molecular weight 80 kDa and 52 kDa
GenBank accession numberBC012390
Gene symbol Factor XII
Gene ID (NCBI) 2161
RRIDAB_2880778
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Factor XII (F XII, Hageman factor) is a 80kD, single chain glycoprotein that circulates in blood as an inactive zymogen. F XII plays an important role in blood coagulation, fibrinolysis, and kinin generation. Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of a 52 kDa NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a 28 kDa COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.

Protocols

Product Specific Protocols
WB protocol for Factor XII antibody 27154-1-APDownload protocol
IHC protocol for Factor XII antibody 27154-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols