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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using Factor XIIIa Polyclonal antibody (17223-1-AP)

    WB analysis of mouse lung using 17223-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 17223-1-AP (Factor XIIIa antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human normal colon using Factor XIIIa Polyclonal antibody (17223-1-AP)

    IHC staining of human normal colon using 17223-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 17223-1-AP (Factor XIIIa antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Factor XIIIa Polyclonal antibody

Factor XIIIa Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17223-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

F13A1, Transglutaminase A chain, Protein-glutamine gamma-glutamyltransferase A chain, F13A, EC:2.3.2.13

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17223-1-AP targets Factor XIIIa in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Factor XIIIa fusion protein Ag11059 相同性解析による交差性が予測される生物種
Full Name coagulation factor XIII, A1 polypeptide
Calculated molecular weight 732 aa, 83 kDa
Observed molecular weight 80-85 kDa
GenBank accession numberBC027963
Gene Symbol Factor XIIIa
Gene ID (NCBI) 2162
RRIDAB_2878363
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00488
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Factor XIIIa is a blood proenzyme that catalyzes intermolecular cross-linking of fibrinogen and is involved in clotting cascade. It has been identified in various tissues including placenta, uterus, and prostate. Anti- Factor XIIIa has been found to be useful in differentiating between dermatofibroma (90% positive) and desmoplastic malignant melanoma (negative). Factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor, and meningioma.

Protocols

Product Specific Protocols
WB protocol for Factor XIIIa antibody 17223-1-APDownload protocol
IHC protocol for Factor XIIIa antibody 17223-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols