F2 Polyclonal antibody
F2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 24295-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human plasma tissue |
| Positive IP detected in | human plasma tissue |
| Positive IF/ICC detected in | HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 3 publications below |
Product Information
24295-1-AP targets F2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, plasmodium falciparum |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | F2 fusion protein Ag18385 相同性解析による交差性が予測される生物種 |
| Full Name | coagulation factor II (prothrombin) |
| Calculated molecular weight | 622 aa, 70 kDa |
| Observed molecular weight | 72 kDa |
| GenBank accession number | BC051332 |
| Gene symbol | F2 |
| Gene ID (NCBI) | 2147 |
| RRID | AB_2879478 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Coagulation Factor II (F2), also known as prothrombin, is one of the components of this chain of plasma proteins involved in blood coagulation. Prothrombin is the precursor of thrombin, which is essential in the processes of hemostasis and thrombosis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for F2 antibody 24295-1-AP | Download protocol |
| IF protocol for F2 antibody 24295-1-AP | Download protocol |
| IP protocol for F2 antibody 24295-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Matrix Biol Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility. | ||
Sci Rep Molecular Camouflage of Plasmodium falciparum Merozoites by Binding of Host Vitronectin to P47 Fragment of SERA5. | ||
Sci Rep C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1. | ||
Malar J The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity. | ||
PLoS Pathog Thrombin cleavage of the hepatitis E virus polyprotein at multiple conserved locations is required for genome replication |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Elke (Verified Customer) (02-23-2023) | it can work on the western blot
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