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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

F2RL3 Polyclonal antibody

F2RL3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25306-1-AP

Synonyms

Proteinase-activated receptor 4, Proteinase activated receptor 4, PAR-4, PAR4, PAR 4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using F2RL3 Polyclonal antibody (25306-1-AP)

    WB analysis using 25306-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25306-1-AP (F2RL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using F2RL3 Polyclonal antibody (25306-1-AP)

    WB analysis of mouse lung using 25306-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 25306-1-AP (F2RL3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using F2RL3 Polyclonal antibody (25306-1-AP)

    WB analysis of mouse pancreas using 25306-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 25306-1-AP (F2RL3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using F2RL3 Polyclonal antibody (25306-1-AP)

    IHC staining of mouse lung using 25306-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 25306-1-AP (F2RL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using F2RL3 Polyclonal antibody (25306-1-AP)

    IHC staining of mouse lung using 25306-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 25306-1-AP (F2RL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse lung tissue, mouse pancreas tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

25306-1-AP targets F2RL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen F2RL3 fusion protein Ag20801 相同性解析による交差性が予測される生物種
Full Name coagulation factor II (thrombin) receptor-like 3
Calculated molecular weight 385 aa, 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC074782
Gene symbol F2RL3
Gene ID (NCBI) 9002
RRIDAB_2880022
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Coagulation factor II receptor-like 3 (F2RL3) encodes a member of the protease-activated receptor subfamily, also known as protease-activated receptor 4 (PAR4), which takes partin platelet activation, intimal hyperplasia and inflammation (PMID:34284820). An absence of PAR4 in mouse models results in impaired hemostasis and a protection against pulmonary embolism, and a small number of missense coding variants in F2RL3 that alter platelet aggregation and function have been described(PMID:35012325). The calculated molecular weight and observed molecular weight of F2RL3 are both 41 kDa.

Protocols

Product Specific Protocols
WB protocol for F2RL3 antibody 25306-1-APDownload protocol
IHC protocol for F2RL3 antibody 25306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oxid Med Cell Longev

Combination of Colchicine and Ticagrelor Inhibits Carrageenan-Induced Thrombi in Mice.

Authors - BuChun Zhang
View Article
mouseWB

Front Oncol

Tumor Suppressor Par-4 Regulates Complement Factor C3 and Obesity.

Authors - Nathalia Araujo
View Article
mouseWB

Neurosci Bull

Antagonism of Protease-Activated Receptor 4 Protects Against Traumatic Brain Injury by Suppressing Neuroinflammation via Inhibition of Tab2/NF-κB Signaling.

Authors - Jianing Luo
View Article