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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

F4/80 Recombinant antibody

F4/80 Recombinant Antibody for IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2P18

Cat no : 81668-1-RR

Synonyms

Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse spleen tissue using F4/80 Recombinant antibody (81668-1-RR)

    IHC staining of mouse spleen using 81668-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using F4/80 Recombinant antibody (81668-1-RR)

    IHC staining of mouse spleen using 81668-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using F4/80 Recombinant antibody (81668-1-RR)

    IHC staining of mouse liver using 81668-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using F4/80 Recombinant antibody (81668-1-RR)

    IHC staining of mouse liver using 81668-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using F4/80 Recombinant antibody (81668-1-RR)

    IF Staining of mouse spleen using 81668-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (81668-1-RR, Clone: 2P18 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using F4/80 Recombinant antibody (81668-1-RR)

    IF Staining of mouse spleen using 81668-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (81668-1-RR, Clone: 2P18 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using F4/80 Recombinant antibody (81668-1-RR)

    IF Staining of RAW 264.7 using 81668-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using F4/80 antibody (81668-1-RR, Clone: 2P18 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 81668-1-RR

    Affinity and Kinetic Characterization of 81668-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81668-1-RR against Mouse F4/80 were performed. The affinity constant is 14.4 nM.

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Tested Applications

Positive IHC detected inmouse spleen tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue, RAW 264.7 cells
Positive IF/ICC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:4000-1:10000
Immunofluorescence (IF)-PIF-P : 1:750-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81668-1-RR targets F4/80 in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen F4/80 fusion protein Ag28252 相同性解析による交差性が予測される生物種
Full Name EGF-like module containing, mucin-like, hormone receptor-like sequence 1
Calculated molecular weight 102 kDa
GenBank accession numberNM_010130
Gene symbol F4/80
Gene ID (NCBI) 13733
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mouse F4/80, also named as EMR1 and Gpf480, is a 160kd cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.

Protocols

Product Specific Protocols
IHC protocol for F4/80 antibody 81668-1-RRDownload protocol
IF protocol for F4/80 antibody 81668-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols