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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

F4/80 Recombinant antibody

F4/80 Recombinant Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241959B6

Cat no : 84538-1-RR

Synonyms

Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of mouse spleen tissue using F4/80 Recombinant antibody (84538-1-RR)

    IHC staining of mouse spleen using 84538-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84538-1-RR (F4/80 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with .

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using F4/80 Recombinant antibody (84538-1-RR)

    IHC staining of mouse spleen using 84538-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84538-1-RR (F4/80 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with .

  • Immunohistochemistry (IHC) staining of mouse liver tissue using F4/80 Recombinant antibody (84538-1-RR)

    IHC staining of mouse liver using 84538-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84538-1-RR (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using F4/80 Recombinant antibody (84538-1-RR)

    IHC staining of mouse liver using 84538-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84538-1-RR (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inmouse spleen tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84538-1-RR targets F4/80 in IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name EGF-like module containing, mucin-like, hormone receptor-like sequence 1
Calculated molecular weight102 kDa
GenBank accession numberNM_010130.4
Gene symbol F4/80
Gene ID (NCBI) 13733
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mouse F4/80, also named as EMR1 and Gpf480, is a 160kd cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.

Protocols

Product Specific Protocols
IHC protocol for F4/80 antibody 84538-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols