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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Mouse F4/80 Rabbit Recombinant Antibody, PBS Only

F4/80 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

241959G4

Cat no : 98236-1-PBS

Synonyms

Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Balb/c mouse peritoneal macrophages using Anti-Mouse F4/80 Rabbit Recombinant Antibody (98236-1-RR)

    FC experiment of Balb/c mouse peritoneal macrophages using 98236-1-RR (same clone as 98236-1-PBS)

    1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse F4/80 Rabbit Recombinant Antibody (98236-1-RR, Clone: 241959G4) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD11b. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98236-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Balb/c mouse peritoneal macrophages using Anti-Mouse F4/80 Rabbit Recombinant Antibody (98236-1-RR)

    FC experiment of Balb/c mouse peritoneal macrophages using 98236-1-RR (same clone as 98236-1-PBS)

    1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse F4/80 Rabbit Recombinant Antibody (98236-1-RR, Clone: 241959G4) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98236-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98236-1-PBS targets F4/80 in FC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name EGF-like module containing, mucin-like, hormone receptor-like sequence 1
Calculated molecular weight102 kDa
GenBank accession numberNM_010130.4
Gene symbol F4/80
Gene ID (NCBI) 13733
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Mouse F4/80, also named as EMR1 and Gpf480, is a 160-kDa cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.