Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | mouse liver tissue, human placenta tissue, human plasma |
| Positive IF/ICC detected in | HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
23058-1-AP targets Factor VII in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Factor VII fusion protein Ag19324 相同性解析による交差性が予測される生物種 |
| Full Name | coagulation factor VII (serum prothrombin conversion accelerator) |
| Calculated molecular weight | 466 aa, 52 kDa |
| Observed molecular weight | 49-55 kDa |
| GenBank accession number | BC130468 |
| Gene Symbol | F7 |
| Gene ID (NCBI) | 2155 |
| RRID | AB_2879203 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | P08709 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
F7(Coagulation factor VII) is also named as serum prothrombin conversion accelerator(SPCA). It is the key triggering enzyme of the extrinsic coagulation pathway. Hepatocytes are the predominant source of F7 production under normal physiologic conditions, although F7 is also expressed by monocytes/macrophages in inflammation and atherosclerosis(PMID:17018600). This protein has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Factor VII antibody 23058-1-AP | Download protocol |
| IF protocol for Factor VII antibody 23058-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Rep C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1. | ||
Drug Des Devel Ther Guanxinning Tablet Alleviates Post-Ischemic Stroke Injury Via Regulating Complement and Coagulation Cascades Pathway and Inflammatory Network Mobilization |