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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

F7 Polyclonal antibody

F7 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23058-1-AP

Synonyms

Factor VII, SPCA, Proconvertin, Eptacog alfa, EC:3.4.21.21

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using F7 Polyclonal antibody (23058-1-AP)

    WB analysis using 23058-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23058-1-AP (F7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using F7 Polyclonal antibody (23058-1-AP)

    WB analysis of human placenta using 23058-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 23058-1-AP (F7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using F7 Polyclonal antibody (23058-1-AP)

    WB analysis of mouse liver using 23058-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 23058-1-AP (F7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using F7 Polyclonal antibody (23058-1-AP)

    WB analysis of human plasma using 23058-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 23058-1-AP (F7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using F7 Polyclonal antibody (23058-1-AP)

    IF Staining of HUVEC using 23058-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using F7 antibody (23058-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inmouse liver tissue, human placenta tissue, human plasma
Positive IF/ICC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

23058-1-AP targets F7 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen F7 fusion protein Ag19324 相同性解析による交差性が予測される生物種
Full Name coagulation factor VII (serum prothrombin conversion accelerator)
Calculated molecular weight 466 aa, 52 kDa
Observed molecular weight 49-55 kDa
GenBank accession numberBC130468
Gene symbol F7
Gene ID (NCBI) 2155
RRIDAB_2879203
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

F7(Coagulation factor VII) is also named as serum prothrombin conversion accelerator(SPCA). It is the key triggering enzyme of the extrinsic coagulation pathway. Hepatocytes are the predominant source of F7 production under normal physiologic conditions, although F7 is also expressed by monocytes/macrophages in inflammation and atherosclerosis(PMID:17018600). This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for F7 antibody 23058-1-APDownload protocol
IF protocol for F7 antibody 23058-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.

Authors - Anika Alberts
View Article
mouseWB

Drug Des Devel Ther

Guanxinning Tablet Alleviates Post-Ischemic Stroke Injury Via Regulating Complement and Coagulation Cascades Pathway and Inflammatory Network Mobilization

Authors - Yule Wang
View Article