Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 15452-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15452-1-AP (FA2H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human stomach using 15452-1-AP
human stomach tissue were subjected to SDS PAGE followed by western blot with 15452-1-AP (FA2H antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 15452-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 15452-1-AP (FA2H antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 15452-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15452-1-AP (FA2H antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 15452-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15452-1-AP (FA2H antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 15452-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15452-1-AP (FA2H antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 15452-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15452-1-AP (FA2H antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 15452-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15452-1-AP (FA2H antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 15452-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15452-1-AP (FA2H antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 15452-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15452-1-AP (FA2H antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 15452-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15452-1-AP (FA2H antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

FA2H Polyclonal antibody

FA2H Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15452-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FAXDC1, Fatty acid hydroxylase domain-containing protein 1, Fatty acid alpha-hydroxylase, Fatty acid 2-hydroxylase, FAAH

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (9)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, human brain tissue, human stomach tissue, mouse colon tissue, MKN-45 cells, SGC-7901 cells, mouse brain tissue
Positive IHC detected in	mouse brain tissue, human colon cancer tissue, rat brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below
IHC	See 2 publications below
IF	See 1 publications below

Product Information

15452-1-AP targets FA2H in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FA2H fusion protein Ag7717 相同性解析による交差性が予測される生物種
Full Name	fatty acid 2-hydroxylase
Calculated molecular weight	43 kDa
Observed molecular weight	38-43 kDa
GenBank accession number	BC002679
Gene Symbol	FA2H
Gene ID (NCBI)	79152
RRID	AB_2101886
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q7L5A8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FA2H(Fatty acid 2-hydroxylase) required for alpha-hydroxylation of free fatty acids and the formation of alpha-hydroxylated sphingolipids. The deduced 372-amino acid protein has a calculated molecular mass of 42.8 kD .Both mouse and human FA2H have a C-terminal endoplasmic reticulum (ER) retention motif(PMID: 15658937).

Protocols

Product Specific Protocols
WB protocol for FA2H antibody 15452-1-AP	Download protocol
IHC protocol for FA2H antibody 15452-1-AP	Download protocol
IF protocol for FA2H antibody 15452-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Cancer Cell Cell Lineage-Based Stratification for Glioblastoma. Authors - Zilai Wang View Article
		Dev Cell Quiescent human glioblastoma cancer stem cells drive tumor initiation, expansion, and recurrence following chemotherapy. Authors - Xuanhua P Xie View Article
	WB	Curr Biol FA2H controls cool temperature sensing through modifying membrane sphingolipids in Drosophila Authors - Qiaoran Li View Article
human	WB,IHC	Front Oncol FA2H Exhibits Tumor Suppressive Roles on Breast Cancers via Cancer Stemness Control. Authors - Xiaofeng Dai View Article
human	WB	J Med Chem Substrate-based fragment identification for the development of selective, nonpeptidic inhibitors of striatal-enriched protein tyrosine phosphatase. Authors - Tyler D Baguley View Article
human	WB	Biochem Biophys Res Commun Fatty acid 2-hydroxylase (FA2H) as a stimulatory molecule responsible for breast cancer cell migration. Authors - Masayo Hirao-Suzuki View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15452-1-AP

WB analysis of human stomach using 15452-1-AP

WB analysis of mouse colon using 15452-1-AP

WB analysis of human brain using 15452-1-AP

IHC staining of mouse brain using 15452-1-AP

IHC staining of rat brain using 15452-1-AP

IHC staining of rat brain using 15452-1-AP

IHC staining of mouse brain using 15452-1-AP

IHC staining of human colon cancer using 15452-1-AP

IHC staining of human colon cancer using 15452-1-AP

IF Staining of HeLa using 15452-1-AP

FA2H Polyclonal antibody

FA2H Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Cell Lineage-Based Stratification for Glioblastoma.

Quiescent human glioblastoma cancer stem cells drive tumor initiation, expansion, and recurrence following chemotherapy.

FA2H controls cool temperature sensing through modifying membrane sphingolipids in Drosophila

FA2H Exhibits Tumor Suppressive Roles on Breast Cancers via Cancer Stemness Control.

Substrate-based fragment identification for the development of selective, nonpeptidic inhibitors of striatal-enriched protein tyrosine phosphatase.

Fatty acid 2-hydroxylase (FA2H) as a stimulatory molecule responsible for breast cancer cell migration.