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KD/KO Validated

FAAH Polyclonal antibody

Name: FAAH Polyclonal antibody
Brand: Proteintech
SKU: 17909-1-AP

FAAH Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 17909-1-AP

Synonyms

Anandamide amidohydrolase 1, FAAH, FAAH 1, FAAH1, fatty acid amide hydrolase, Fatty acid amide hydrolase 1, Oleamide hydrolase 1

Validation Data Gallery

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AllWBIPIHC

WB analysis using 17909-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 17909-1-AP (FAAH antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 17909-1-AP
WB result of FAAH antibody (17909-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAAH transfected A431 cells.
WB analysis of rat testis using 17909-1-AP
rat testis tissue were subjected to SDS PAGE followed by western blot with 17909-1-AP (FAAH Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 17909-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 17909-1-AP (FAAH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 17909-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 17909-1-AP (FAAH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 17909-1-AP
IP result of anti-FAAH (IP:17909-1-AP, 4ug; Detection:17909-1-AP 1:500) with mouse liver tissue lysate 6400 ug.
IHC staining of mouse testis using 17909-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17909-1-AP (FAAH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 17909-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17909-1-AP (FAAH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 17909-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17909-1-AP (FAAH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 17909-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17909-1-AP (FAAH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 17909-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17909-1-AP (FAAH Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 17909-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17909-1-AP (FAAH Antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 17909-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse liver tissue, rat testis tissue, A431 cells
Positive IP detected in	mouse liver tissue
Positive IHC detected in	mouse testis tissue, human placenta tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:300
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 6 publications below
IP	See 1 publications below

Product Information

The immunogen of 17909-1-AP is FAAH Fusion Protein expressed in E. coli.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FAAH fusion protein Ag12304 相同性解析による交差性が予測される生物種
Full Name	fatty acid amide hydrolase
Calculated molecular weight	579 aa, 63 kDa
Observed molecular weight	55-65 kDa
GenBank accession number	BC093632
Gene symbol	FAAH
Gene ID (NCBI)	2166
RRID	AB_2878465
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Fatty acid amide hydrolase (FAAH) is a membrane-bound homodimeric enzyme that in vivo controls content and biological activity of N-arachidonoylethanolamine (AEA) and other relevant bioactive lipids termed endocannabinoids. (PMID: 32041998) This antibody is specific for FAAH protein.

Protocols

Product Specific Protocols
WB protocol for FAAH antibody 17909-1-AP	Download protocol
IHC protocol for FAAH antibody 17909-1-AP	Download protocol
IP protocol for FAAH antibody 17909-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Elife p21-activated kinase 1 restricts tonic endocannabinoid signaling in the hippocampus. Authors - Shuting Xia View Article
rat	WB	J Proteome Res Joint Synovial Fluid Metabolomics Method to Decipher the Metabolic Mechanisms of Adjuvant Arthritis and Geniposide Intervention. Authors - Xiang Zhan View Article
mouse	WB	Neuroscience The Endocannabinoid System Contributes to Memory Deficits Induced by Rapid-eye-movement Sleep Deprivation in Adolescent Mice. Authors - Jinming Zhang View Article
human,mouse	WB,IP	Cell Death Differ FAAH served a key membrane-anchoring and stabilizing role for NLRP3 protein independently of the endocannabinoid system Authors - Yangyang Zhu KO Validated View Article
human	WB	Cell Death Dis Combination treatment with FAAH inhibitors/URB597 and ferroptosis inducers significantly decreases the growth and metastasis of renal cell carcinoma cells via the PI3K-AKT signaling pathway Authors - Junfeng Hao View Article
mouse	WB	Biochim Biophys Acta Mol Basis Dis Inactivation of fatty acid amide hydrolase protects against ischemic reperfusion injury-induced renal fibrogenesis Authors - Chaoling Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAAH Polyclonal antibody

FAAH Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 17909-1-AP

WB analysis of A431 using 17909-1-AP

WB analysis of rat testis using 17909-1-AP

WB analysis of mouse liver using 17909-1-AP

WB analysis of A431 using 17909-1-AP

IP experiment of mouse liver using 17909-1-AP

IHC staining of mouse testis using 17909-1-AP

IHC staining of mouse testis using 17909-1-AP

IHC staining of human placenta using 17909-1-AP

IHC staining of human placenta using 17909-1-AP

IHC staining of human testis using 17909-1-AP

IHC staining of human testis using 17909-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

p21-activated kinase 1 restricts tonic endocannabinoid signaling in the hippocampus.

Joint Synovial Fluid Metabolomics Method to Decipher the Metabolic Mechanisms of Adjuvant Arthritis and Geniposide Intervention.

The Endocannabinoid System Contributes to Memory Deficits Induced by Rapid-eye-movement Sleep Deprivation in Adolescent Mice.

FAAH served a key membrane-anchoring and stabilizing role for NLRP3 protein independently of the endocannabinoid system

Combination treatment with FAAH inhibitors/URB597 and ferroptosis inducers significantly decreases the growth and metastasis of renal cell carcinoma cells via the PI3K-AKT signaling pathway

Inactivation of fatty acid amide hydrolase protects against ischemic reperfusion injury-induced renal fibrogenesis