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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAAH Monoclonal antibody, PBS Only

FAAH Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, rabbit, pig, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3C5B5

Cat no : 67607-1-PBS

Synonyms

Anandamide amidohydrolase 1, FAAH, FAAH 1, FAAH1, fatty acid amide hydrolase, Fatty acid amide hydrolase 1, Oleamide hydrolase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAAH Monoclonal antibody (67607-1-Ig)

    WB analysis using 67607-1-Ig (same clone as 67607-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67607-1-Ig (FAAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of JAR cells using FAAH Monoclonal antibody (67607-1-Ig)

    WB analysis of JAR using 67607-1-Ig (same clone as 67607-1-PBS)

    JAR cells were subjected to SDS PAGE followed by western blot with 67607-1-Ig (FAAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using FAAH Monoclonal antibody (67607-1-Ig)

    WB analysis of LNCaP using 67607-1-Ig (same clone as 67607-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67607-1-Ig (FAAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FAAH Monoclonal antibody (67607-1-Ig)

    IHC staining of human liver cancer using 67607-1-Ig (same clone as 67607-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FAAH Monoclonal antibody (67607-1-Ig)

    IHC staining of human liver cancer using 67607-1-Ig (same clone as 67607-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 410x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FAAH Monoclonal antibody (67607-1-Ig)

    IHC staining of human prostate cancer using 67607-1-Ig (same clone as 67607-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FAAH Monoclonal antibody (67607-1-Ig)

    IHC staining of human prostate cancer using 67607-1-Ig (same clone as 67607-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using FAAH Monoclonal antibody (67607-1-Ig)

    IF Staining of LNCaP using 67607-1-Ig (same clone as 67607-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using FAAH antibody (67607-1-Ig, Clone: 3C5B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67607-1-PBS targets FAAH in WB, IF, IHC, ELISA applications and shows reactivity with Human, rat, rabbit, pig, mouse samples.

Tested Reactivity Human, rat, rabbit, pig, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FAAH fusion protein Ag12720 相同性解析による交差性が予測される生物種
Full Name fatty acid amide hydrolase
Calculated molecular weight 579 aa, 63 kDa
Observed molecular weight55-65 kDa
GenBank accession numberBC093632
Gene symbol FAAH
Gene ID (NCBI) 2166
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Fatty acid amide hydrolase (FAAH) is a membrane-bound homodimeric enzyme that in vivo controls content and biological activity of N-arachidonoylethanolamine (AEA) and other relevant bioactive lipids termed endocannabinoids. (PMID: 32041998) This antibody is specific for FAAH protein.