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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FABP1 Monoclonal antibody (68227-1-Ig)

    WB analysis using 68227-1-Ig (same clone as 68227-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68227-1-Ig (FABP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using FABP1 Monoclonal antibody (68227-1-Ig)

    WB analysis of pig liver using 68227-1-Ig (same clone as 68227-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68227-1-Ig (FABP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using FABP1 Monoclonal antibody (68227-1-Ig)

    FC experiment of HepG2 using 68227-1-Ig (same clone as 68227-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug FABP1 Monoclonal antibody (68227-1-Ig, Clone:1B6C8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

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FABP1 Monoclonal antibody, PBS Only

FABP1 Monoclonal Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 68227-1-PBS
Clone No.1B6C8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

liver FABP, L-FABP, Fatty acid-binding protein, liver, Fatty acid-binding protein 1, Fatty acid binding protein 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68227-1-PBS targets FABP1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FABP1 fusion protein Ag32836 相同性解析による交差性が予測される生物種
Full Name fatty acid binding protein 1, liver
Calculated molecular weight 127 aa, 14 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC032801
Gene Symbol FABP1
Gene ID (NCBI) 2168
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP07148
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FABP1, liver fatty acid-binding protein, is abundant in cytoplasm that regulates lipid transport and metabolism. It plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (PMID:25732850). FABP1 is mainly expressed in hepatocytes, enterocytes and to a lesser degree in renal tubular cells, associated with liver injury (PMID: 15653098). Levels of FABP1 was elevated in patients with hepatocyte injury secondary to alcohol or drug toxicity (PMID: 14563446).