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FABP2 Monoclonal antibody

FABP2 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2D11G6

Cat no : 67691-1-Ig

Synonyms

FABP2, FABPI, Fatty acid binding protein 2, I FABP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FABP2 Monoclonal antibody (67691-1-Ig)

    WB analysis using 67691-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67691-1-Ig (FABP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using FABP2 Monoclonal antibody (67691-1-Ig)

    WB analysis of COLO 320 using 67691-1-Ig

    WB result of FABP2 antibody (67691-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FABP2 transfected COLO 320 cells.

  • Western Blot (WB) analysis of various lysates using FABP2 Monoclonal antibody (67691-1-Ig)

    WB analysis using 67691-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67691-1-Ig (FABP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using FABP2 Monoclonal antibody (67691-1-Ig)

    IHC staining of rat small intestine using 67691-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using FABP2 Monoclonal antibody (67691-1-Ig)

    IHC staining of human small intestine using 67691-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using FABP2 Monoclonal antibody (67691-1-Ig)

    IHC staining of human small intestine using 67691-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using FABP2 Monoclonal antibody (67691-1-Ig)

    IHC staining of mouse small intestine using 67691-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using FABP2 Monoclonal antibody (67691-1-Ig)

    IHC staining of mouse small intestine using 67691-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using FABP2 Monoclonal antibody (67691-1-Ig)

    IHC staining of mouse colon using 67691-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using FABP2 Monoclonal antibody (67691-1-Ig)

    IF Staining of mouse colon using 67691-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using FABP2 antibody (67691-1-Ig, Clone: 2D11G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inrat small intestine tissue, human jejunum tissue, COLO 320 cells, pig duodenum, mouse small intestine, rabbit small intestine
Positive IHC detected inmouse small intestine tissue, mouse colon tissue, rat small intestine tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse colon tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67691-1-Ig targets FABP2 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Reactivity Human, mouse, rat, rabbit, pig
Cited Reactivity mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FABP2 fusion protein Ag17620 相同性解析による交差性が予測される生物種
Full Name fatty acid binding protein 2, intestinal
Calculated molecular weight 132 aa, 15 kDa
Observed molecular weight15 kDa
GenBank accession numberBC069617
Gene symbol FABP2
Gene ID (NCBI) 2169
RRIDAB_2882884
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FABP2, also known as the intestinal fatty acid binding protein (I-FABP), is expressed in the absorptive intestinal villus cells. It is mainly involved in intracellular transport and intestinal absorption of lipids. FABP2 has been considered a marker of mucosal injury and ischemia and serum I-FABP level is used as a tissue damage indicator. In addition, it is a marker of differentiated intestinal epithelial cells.

Protocols

Product Specific Protocols
WB protocol for FABP2 antibody 67691-1-IgDownload protocol
IHC protocol for FABP2 antibody 67691-1-IgDownload protocol
IF protocol for FABP2 antibody 67691-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

Anti-Proteolytic Peptide R7I Protects the Intestinal Barrier and Alleviates Fatty Acid Malabsorption in Salmonella typhimurium-Infected Mice

Authors - Yunzhe Su
View Article