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  • Featured Product
  • KD/KO Validated

FABP5 Monoclonal antibody, PBS Only

FABP5 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C6E12

Cat no : 66299-1-PBS

Synonyms

E FABP, EFABP, FABP5, Fatty acid binding protein 5, PA FABP, PAFABP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FABP5 Monoclonal antibody (66299-1-Ig)

    WB analysis using 66299-1-Ig (same clone as 66299-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66299-1-Ig (FABP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66299-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using FABP5 Monoclonal antibody (66299-1-Ig)

    WB analysis of A375 using 66299-1-Ig (same clone as 66299-1-PBS)

    WB result of FABP5 antibody (66299-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FABP5 transfected A375 cells. This data was developed using the same antibody clone with 66299-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using FABP5 Monoclonal antibody (66299-1-Ig)

    WB analysis of A375 using 66299-1-Ig (same clone as 66299-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 66299-1-Ig (FABP5 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66299-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FABP5 Monoclonal antibody (66299-1-Ig)

    WB analysis using 66299-1-Ig (same clone as 66299-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66299-1-Ig (FABP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66299-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using FABP5 Monoclonal antibody (66299-1-Ig)

    WB analysis of fetal human brain using 66299-1-Ig (same clone as 66299-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66299-1-Ig (FABP5 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66299-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brown adipose tissue using FABP5 Monoclonal antibody (66299-1-Ig)

    IHC staining of mouse brown adipose using 66299-1-Ig (same clone as 66299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 66299-1-Ig (FABP5 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66299-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brown adipose tissue using FABP5 Monoclonal antibody (66299-1-Ig)

    IHC staining of mouse brown adipose using 66299-1-Ig (same clone as 66299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 66299-1-Ig (FABP5 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66299-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FABP5 Monoclonal antibody (66299-1-Ig)

    IHC staining of human breast cancer using 66299-1-Ig (same clone as 66299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66299-1-Ig (FABP5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66299-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FABP5 Monoclonal antibody (66299-1-Ig)

    IHC staining of human breast cancer using 66299-1-Ig (same clone as 66299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66299-1-Ig (FABP5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66299-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FABP5 Monoclonal antibody (66299-1-Ig)

    IHC staining of human prostate cancer using 66299-1-Ig (same clone as 66299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66299-1-Ig (FABP5 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66299-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FABP5 Monoclonal antibody (66299-1-Ig)

    IHC staining of human prostate cancer using 66299-1-Ig (same clone as 66299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66299-1-Ig (FABP5 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66299-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FABP5 Monoclonal antibody (66299-1-Ig)

    IF Staining of HepG2 using 66299-1-Ig (same clone as 66299-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using FABP5 antibody (66299-1-Ig, Clone: 1C6E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66299-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using FABP5 Monoclonal antibody (66299-1-Ig)

    FC experiment of HeLa using 66299-1-Ig (same clone as 66299-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.20 ug/test Anti-Human FABP5 (66299-1-Ig, Clone:1C6E12) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Fixed with 90% MeOH. This data was developed using the same antibody clone with 66299-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66299-1-PBS targets FABP5 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FABP5 fusion protein Ag3005 相同性解析による交差性が予測される生物種
Full Name fatty acid binding protein 5 (psoriasis-associated)
Calculated molecular weight 135 aa, 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC019385
Gene symbol FABP5
Gene ID (NCBI) 2171
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

FABP5, also named as PA-FABP and E-FABP, belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. It is high specificity for fatty acids. FABP5 is highest affinity for C18 chain length. It may be involved in keratinocyte differentiation. FABP5 is a fatty acid-binding protein and is expressed in epidermis and endothelial cells of the microvasculature of different organs. FABP5 has also been identified as a tumor-associated antigen, which is highly expressed in various cancers. FABP5 was detected in the sera of HNSCC patients with early stage cancer. Antibodies specific for FABP5 were significantly increased in a substantial amount in patients, suggesting that FABP5 may be a potential diagnostic biomarker for HNSCC. FABP5 may serve as a biomarker for HNSCC.(PMID:19602232)