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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FABP7-Specific Polyclonal antibody

FABP7-Specific Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 14836-1-AP

Synonyms

FABP7, Brain-type fatty acid-binding protein, Brain lipid-binding protein, BLBP, B-FABP

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using FABP7-Specific Polyclonal antibody (14836-1-AP)

    WB analysis of human brain using 14836-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14836-1-AP (FABP7-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-251 cells using FABP7-Specific Polyclonal antibody (14836-1-AP)

    WB analysis of U-251 using 14836-1-AP

    U-251 cells were subjected to SDS PAGE followed by western blot with 14836-1-AP (FABP7-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FABP7-Specific Polyclonal antibody (14836-1-AP)

    IHC staining of human breast cancer using 14836-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14836-1-AP (FABP7-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FABP7-Specific Polyclonal antibody (14836-1-AP)

    IHC staining of human breast cancer using 14836-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14836-1-AP (FABP7-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using FABP7-Specific Polyclonal antibody (14836-1-AP)

    IF Staining of rat brain using 14836-1-AP

    Immunofluorescent analysis of (-20°C Acetone) fixed rat brain tissue using FABP7-Specific antibody (14836-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using FABP7-Specific Polyclonal antibody (14836-1-AP)

    IF Staining of rat brain using 14836-1-AP

    Immunofluorescent analysis of (-20°C Acetone) fixed rat brain tissue using FABP7-Specific antibody (14836-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inhuman brain tissue, U-251 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14836-1-AP targets FABP7-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name fatty acid binding protein 7, brain
Calculated molecular weight 15 kDa
Observed molecular weight 14-15 kDa
GenBank accession numberNM_001446
Gene symbol FABP7
Gene ID (NCBI) 2173
RRIDAB_2100458
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FABP7 (Fatty Acid Binding Protein-7), also known as B-FABP (Brain Fatty Acid-Binding Protein), is a member of FABP family which are intracellular proteins involved in lipid metabolism. Normally FABP7 is mainly expressed in brain and other neural tissues and is important in early nervous system development. In addition, FABP7 is considered a marker for some brain tumors and may have a role in tumorigenesis in the peripheral nervous system and in several extra nervous system neoplasms such as breast cancer, renal cancer, and melanoma. This antibody specially recognizes FABP7 and not cross-react with other FABPs.

Protocols

Product Specific Protocols
WB protocol for FABP7-Specific antibody 14836-1-APDownload protocol
IHC protocol for FABP7-Specific antibody 14836-1-APDownload protocol
IF protocol for FABP7-Specific antibody 14836-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Nucleic Acids Res

H2A.Z.1 crosstalk with H3K56-acetylation controls gliogenesis through the transcription of folate receptor.

Authors - Libo Su
View Article
humanIHC

Ther Adv Med Oncol

Development and external validation of a composite immune-clinical prognostic model associated with EGFR mutation in East-Asian patients with lung adenocarcinoma.

Authors - Chengming Liu
View Article