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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FADD Recombinant antibody

FADD Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241976A11

Cat no : 84619-5-RR

Synonyms

MORT1, Growth-inhibiting gene 3 protein, FAS-associating death domain-containing protein, FAS-associated death domain protein, 241976A11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FADD Recombinant antibody (84619-5-RR)

    WB analysis using 84619-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84619-5-RR (FADD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FADD Recombinant antibody (84619-5-RR)

    IF Staining of HeLa using 84619-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FADD antibody (84619-5-RR, Clone: 241976A11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A431 cells using FADD Recombinant antibody (84619-5-RR)

    FC experiment of A431 using 84619-5-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug FADD Recombinant antibody (84619-5-RR, Clone:241976A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HT-1080 cells using FADD Recombinant antibody (84619-5-RR)

    FC experiment of HT-1080 using 84619-5-RR

    1x10^6 HT-1080 cells were intracellularly stained with 0.25 ug FADD Recombinant antibody (84619-5-RR, Clone:241976A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84619-5-RR

    Affinity and Kinetic Characterization of 84619-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84619-5-RR against Human FADD were performed. The affinity constant is 19.8 nM.

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA431 cells, HT-1080 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84619-5-RR targets FADD in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FADD fusion protein Ag6701 相同性解析による交差性が予測される生物種
Full Name Fas (TNFRSF6)-associated via death domain
Calculated molecular weight 23 kDa
Observed molecular weight29 kDa
GenBank accession numberBC000334
Gene symbol FADD
Gene ID (NCBI) 8772
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fas-Associated protein with Death Domain (FADD), also called MORT1 or GIG3, is encoded by the FADD gene. FADD is an adaptor protein that bridges members of the tumor necrosis factor receptor superfamily, such as the Fas-receptor, to procaspases 8 and 10 to form the death-inducing signaling complex (DISC) during apoptosis. As well as its most well known role in apoptosis, FADD has also been seen to play a role in other processes including proliferation, cell cycle regulation and development. FADD has a calculated molecular mass of 23 kDa and always can be detected as 23-30 kDa (PMID: 15390286, 22864571, 17977957)

Protocols

Product Specific Protocols
WB protocol for FADD antibody 84619-5-RRDownload protocol
IF protocol for FADD antibody 84619-5-RRDownload protocol
FC protocol for FADD antibody 84619-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols