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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using FADS2 Monoclonal antibody (68026-1-Ig)

    WB analysis of PC-3 using 68026-1-Ig (same clone as 68026-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68026-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using FADS2 Monoclonal antibody (68026-1-Ig)

    WB analysis of LNCaP using 68026-1-Ig (same clone as 68026-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68026-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using FADS2 Monoclonal antibody (68026-1-Ig)

    WB analysis of U2OS using 68026-1-Ig (same clone as 68026-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68026-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using FADS2 Monoclonal antibody (68026-1-Ig)

    WB analysis of Jurkat using 68026-1-Ig (same clone as 68026-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68026-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using FADS2 Monoclonal antibody (68026-1-Ig)

    WB analysis of rat liver using 68026-1-Ig (same clone as 68026-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68026-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using FADS2 Monoclonal antibody (68026-1-Ig)

    WB analysis of A549 using 68026-1-Ig (same clone as 68026-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68026-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using FADS2 Monoclonal antibody (68026-1-Ig)

    IHC staining of mouse liver using 68026-1-Ig (same clone as 68026-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68026-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using FADS2 Monoclonal antibody (68026-1-Ig)

    IHC staining of mouse liver using 68026-1-Ig (same clone as 68026-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68026-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using FADS2 Monoclonal antibody (68026-1-Ig)

    IF Staining of A549 using 68026-1-Ig (same clone as 68026-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using FADS2 antibody (68026-1-Ig, Clone: 1G4B10 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68026-1-PBS in a different storage buffer formulation.

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FADS2 Monoclonal antibody, PBS Only

FADS2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68026-1-PBS
Clone No.1G4B10

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EC:1.14.19.3, Delta-6 desaturase, D6D, Acyl-CoA 6-desaturase, 1G4B10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68026-1-PBS targets FADS2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FADS2 fusion protein Ag27715 相同性解析による交差性が予測される生物種
Full Name fatty acid desaturase 2
Calculated molecular weight 445 aa, 49 kDa
Observed molecular weight42-45 kDa
GenBank accession numberBC009011
Gene Symbol FADS2
Gene ID (NCBI) 9415
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95864
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Fatty acid desaturase 2 (FADS2) is responsible for the first desaturation reaction in the synthesis of highly unsaturated fatty acids (HUFAs), such as arachidonic acid (20:4n-6) and eicosapentaenoic acid (20:5n-3), and is involved in Mead acid (20:3n-9) production during essential fatty acid deficiency (EFAD) (PMID: 29353041). This is important when temperatures changes and the membrane is under distress. It has 4 isoforms produced by alternative splicing.