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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FADS2-Specific Recombinant antibody

FADS2-Specific Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241863D2

Cat no : 84527-4-RR

Synonyms

FADS2, EC:1.14.19.3, Delta-6 desaturase, D6D, Acyl-CoA 6-desaturase

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of L02 cells using FADS2-Specific Recombinant antibody (84527-4-RR)

    WB analysis of L02 using 84527-4-RR

    L02 cells were subjected to SDS PAGE followed by western blot with 84527-4-RR (FADS2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FADS2-Specific Recombinant antibody (84527-4-RR)

    WB analysis using 84527-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84527-4-RR (FADS2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FADS2-Specific Recombinant antibody (84527-4-RR)

    WB analysis using 84527-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84527-4-RR (FADS2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inL02 cells, Jurkat cells, mouse liver tissue, LNCaP cells, A549 cells, PC-3 cells, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84527-4-RR is FADS2-Specific Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FADS2-Specific fusion protein Ag27715 相同性解析による交差性が予測される生物種
Full Name fatty acid desaturase 2
Calculated molecular weight 445 aa, 49 kDa
Observed molecular weight45 kDa
GenBank accession numberBC009011
Gene symbol FADS2
Gene ID (NCBI) 9415
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fatty acid desaturase 2 (FADS2) is responsible for the first desaturation reaction in the synthesis of highly unsaturated fatty acids (HUFAs), such as arachidonic acid (20:4n-6) and eicosapentaenoic acid (20:5n-3), and is involved in Mead acid (20:3n-9) production during essential fatty acid deficiency (EFAD) (PMID: 29353041). This is important when temperatures changes and the membrane is under distress. It has 4 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for FADS2-Specific antibody 84527-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols