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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using FADS2 Recombinant monoclonal antibody (84527-5-RR)

    IF Staining of U2OS using 84527-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FADS2 antibody (84527-5-RR, Clone: 241863G3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using FADS2 Recombinant monoclonal antibody (84527-5-RR)

    IF Staining of L02 using 84527-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using FADS2 antibody (84527-5-RR, Clone: 241863G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HepG2 cells using FADS2 Recombinant monoclonal antibody (84527-5-RR)

    FC experiment of HepG2 using 84527-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug FADS2 Recombinant monoclonal antibody (84527-5-RR, Clone:241863G3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • Affinity and Kinetic Characterization of 84527-5-RR

    Affinity and Kinetic Characterization of 84527-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84527-5-RR against Human FADS2-Specific were performed. The affinity constant is below 1 pM.

FADS2 Recombinant monoclonal antibody

FADS2 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 84527-5-RR
Clone No.241863G3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

241863G3, Acyl-CoA 6-desaturase, D6D, Delta-6 desaturase, EC:1.14.19.3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inU2OS cells, L02 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84527-5-RR targets FADS2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag27715

Product name: Recombinant human FADS2-Specific protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-124 aa of BC009011

Sequence: MGKGGNQGEGAAEREVSVPTFSWEEIQKHNLRTDRWLVIDRKVYNITKWSIQHPGGQRVIGHYAGEDATDAFRAFHPDLEFVGKFLKPLLIGELAPEEPSQDHGKNSKITEDFRALRKTAEDMN

相同性解析による交差性が予測される生物種
Full Name fatty acid desaturase 2
Calculated molecular weight 445 aa, 49 kDa
GenBank accession numberBC009011
Gene Symbol FADS2
Gene ID (NCBI) 9415
RRIDAB_3672035
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDO95864
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fatty acid desaturase 2 (FADS2) is responsible for the first desaturation reaction in the synthesis of highly unsaturated fatty acids (HUFAs), such as arachidonic acid (20:4n-6) and eicosapentaenoic acid (20:5n-3), and is involved in Mead acid (20:3n-9) production during essential fatty acid deficiency (EFAD) (PMID: 29353041). This is important when temperatures changes and the membrane is under distress. It has 4 isoforms and can be detected as 46-52 kDa.

Protocols

Product Specific Protocols
FC protocol for FADS2 antibody 84527-5-RRDownload protocol
IF protocol for FADS2 antibody 84527-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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