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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FADS3 Polyclonal antibody

FADS3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15205-1-AP

Synonyms

fatty acid desaturase 3, FADS 3, EC:1.14.19.-, Delta(13) fatty acid desaturase (Delta(13) desaturase ), Cytochrome b5 related protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FADS3 Polyclonal antibody (15205-1-AP)

    WB analysis using 15205-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FADS3 Polyclonal antibody (15205-1-AP)

    WB analysis using 15205-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FADS3 Polyclonal antibody (15205-1-AP)

    WB analysis using 15205-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using FADS3 Polyclonal antibody (15205-1-AP)

    WB analysis of mouse kidney using 15205-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of L02 cells using FADS3 Polyclonal antibody (15205-1-AP)

    IP experiment of L02 using 15205-1-AP

    IP result of anti-FADS3 (IP:15205-1-AP, 4ug; Detection:15205-1-AP 1:500) with L02 cells lysate 3000 ug.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using FADS3 Polyclonal antibody (15205-1-AP)

    IHC staining of human ovary cancer using 15205-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15205-1-AP (FADS3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, mouse skeletal muscle tissue, mouse kidney tissue, L02 cells, rat skeletal muscle tissue, mouse liver tissue
Positive IP detected inL02 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15205-1-AP targets FADS3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FADS3 fusion protein Ag7355 相同性解析による交差性が予測される生物種
Full Name fatty acid desaturase 3
Calculated molecular weight 51 kDa
Observed molecular weight 41-51 kDa
GenBank accession numberBC004901
Gene symbol FADS3
Gene ID (NCBI) 3995
RRIDAB_10859776
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FADS3, also named as CYB5RP, belongs to fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family memebers are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase protion, both of which are characterized by conserved histidine motifs. FADS3 can be detected as 40 kDa-51 kDa.

Protocols

Product Specific Protocols
WB protocol for FADS3 antibody 15205-1-APDownload protocol
IHC protocol for FADS3 antibody 15205-1-APDownload protocol
IP protocol for FADS3 antibody 15205-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols