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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using FAF1 Monoclonal antibody (60231-1-Ig)

    WB analysis of HEK-293 using 60231-1-Ig (same clone as 60231-1-PBS)

    WB result of FAF1 antibody (60231-1-Ig, 1:5000) with si-Control and si-FAF1 transfected HEK-293 cells. This data was developed using the same antibody clone with 60231-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using FAF1 Monoclonal antibody (60231-1-Ig)

    WB analysis of HEK-293 using 60231-1-Ig (same clone as 60231-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60231-1-Ig (FAF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60231-1-PBS in a different storage buffer formulation.

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FAF1 Monoclonal antibody, PBS Only

FAF1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 60231-1-PBS
Clone No.2E1D7

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

CGI 03, FAF1, FAS associated factor 1, hFAF1, HFAF1s, UBXD12, UBXN3A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60231-1-PBS targets FAF1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FAF1 fusion protein Ag0407 相同性解析による交差性が予測される生物種
Full Name Fas (TNFRSF6) associated factor 1
Calculated molecular weight650aa, 74 kDa
Observed molecular weight 74 kDa
GenBank accession numberBC004970
Gene Symbol FAF1
Gene ID (NCBI) 11124
RRIDAB_11232212
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UNN5
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

FAF1 was detected most abundant in testis, slightly less abundant in skeletal muscle and heart, followed by prostate, thymus, ovary, small intestine, and colon, but not in the peripheral blood leukocytes.The N-terminal region (amino acid 1∼201) including the upstream ubiquitin homology domain of hFAF1 could bind with the death domain of Fas, which mediates programmed cell death, also called apoptosis, in a number of organ systems, notably the immune and nervous systems.