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  • Featured Product
  • KD/KO Validated

FAK Polyclonal antibody, PBS Only

FAK Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12636-1-PBS

Synonyms

PTK2, p125FAK, Focal adhesion kinase-related nonkinase, Focal adhesion kinase 1, FAK1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAK Polyclonal antibody (12636-1-AP)

    WB analysis using 12636-1-AP (same clone as 12636-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12636-1-AP (FAK antibody) at dilution of 1:70000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12636-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using FAK Polyclonal antibody (12636-1-AP)

    WB analysis of NIH/3T3 using 12636-1-AP (same clone as 12636-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12636-1-AP (FAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12636-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using FAK Polyclonal antibody (12636-1-AP)

    IP experiment of HeLa using 12636-1-AP (same clone as 12636-1-PBS)

    IP result of anti-FAK (IP:12636-1-AP, 4ug; Detection:12636-1-AP 1:30000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 12636-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using FAK Polyclonal antibody (12636-1-AP)

    IHC staining of human gliomas using 12636-1-AP (same clone as 12636-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 12636-1-AP (FAK antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12636-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FAK Polyclonal antibody (12636-1-AP)

    IHC staining of human breast cancer using 12636-1-AP (same clone as 12636-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12636-1-AP (FAK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12636-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FAK Polyclonal antibody (12636-1-AP)

    IHC staining of human breast cancer using 12636-1-AP (same clone as 12636-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12636-1-AP (FAK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12636-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using FAK Polyclonal antibody (12636-1-AP)

    IF Staining of A549 using 12636-1-AP (same clone as 12636-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using FAK antibody (12636-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 12636-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using FAK Polyclonal antibody (12636-1-AP)

    IF Staining of HUVEC using 12636-1-AP (same clone as 12636-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using FAK antibody (12636-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 12636-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12636-1-PBS targets FAK in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAK fusion protein Ag3331 相同性解析による交差性が予測される生物種
Full Name PTK2 protein tyrosine kinase 2
Calculated molecular weight 1052 aa, 119 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC028733
Gene symbol FAK
Gene ID (NCBI) 5747
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.