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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OTULIN Polyclonal antibody (27392-1-AP)

    WB analysis using 27392-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27392-1-AP (OTULIN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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OTULIN Polyclonal antibody

OTULIN Polyclonal Antibody for WB, ELISA
Cat No. 27392-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

FAM105B, OTULIN, Protein FAM105B, Ubiquitin thioesterase otulin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27392-1-AP targets OTULIN in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OTULIN fusion protein Ag22302 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 105, member B
Calculated molecular weight 352 aa, 40 kDa
Observed molecular weight40-50 kDa
GenBank accession numberBC007706
Gene Symbol FAM105B
Gene ID (NCBI) 90268
RRIDAB_3085952
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96BN8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Linear ubiquitination is negatively regulated by OTULIN (OTU deubiquitinase with linear linkage specificity, also known as Fam105b or Gumby), a deubiquitinase specifically catalysing the degradation of linear ubiquitin chains. OUb signalling is antagonised by deubiquitinases (DUBs), which cleave the polyUb signal from substrates to terminate signalling. OTU DUB with linear linkage specificity (OTULIN) and CYLD are the two main DUBs that regulate M1-polyUb signalling. OTULIN exclusively cleaves M1 linkages, whereas CYLD cleaves both M1 and K63 linkages. OTULIN binds directly to the LUBAC subunit HOIP and regulates LUBAC signalling, autoubiquitination, and stability.(PMID: 34625557, PMID: 32231246)

Protocols

Product Specific Protocols
WB protocol for OTULIN antibody 27392-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols