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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAM114A1 Monoclonal antibody (67926-1-Ig)

    WB analysis using 67926-1-Ig (same clone as 67926-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67926-1-Ig (FAM114A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67926-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using FAM114A1 Monoclonal antibody (67926-1-Ig)

    WB analysis of NIH/3T3 using 67926-1-Ig (same clone as 67926-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67926-1-Ig (FAM114A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67926-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using FAM114A1 Monoclonal antibody (67926-1-Ig)

    WB analysis of U2OS using 67926-1-Ig (same clone as 67926-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 67926-1-Ig (FAM114A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67926-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using FAM114A1 Monoclonal antibody (67926-1-Ig)

    WB analysis of PC-12 using 67926-1-Ig (same clone as 67926-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67926-1-Ig (FAM114A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67926-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FAM114A1 Monoclonal antibody (67926-1-Ig)

    IHC staining of mouse brain using 67926-1-Ig (same clone as 67926-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67926-1-Ig (FAM114A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67926-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FAM114A1 Monoclonal antibody (67926-1-Ig)

    IF Staining of HepG2 using 67926-1-Ig (same clone as 67926-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FAM114A1 antibody (67926-1-Ig, Clone: 1B11D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67926-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51192-1

    Cytometric bead array standard curve of MP51192-1

    Cytometric bead array standard curve of MP51192-1, FAM114A1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67926-1-PBS. Detection antibody: 67926-2-PBS. Standard:Ag17289. Range: 0.195-50 ng/mL.

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FAM114A1 Monoclonal antibody, PBS Only (Capture)

FAM114A1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 67926-1-PBS
Clone No.1B11D6

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

Protein NOXP20, Protein FAM114A1, NOXP20, Nervous system overexpressed protein 20, 1B11D6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67926-1-PBS targets FAM114A1 as part of a matched antibody pair:

MP51192-1: 67926-1-PBS capture and 67926-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen FAM114A1 fusion protein Ag17289 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 114, member A1
Calculated molecular weight 563 aa, 61 kDa
Observed molecular weight80 kDa
GenBank accession numberBC040452
Gene Symbol FAM114A1
Gene ID (NCBI) 92689
RRIDAB_2918678
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IWE2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

FAM114A1, also named as Nervous system overexpressed protein 20, is a 563 amino acid protein, which belongs to the FAM114 family. FAM114A1 localizes in the cytoplasm and may play a role in neuronal cell development. The calculated molecular weight of FAM114A1 is 61 kDa, but the modified FAM114A1 may be 70-80 kDa protein.