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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAM131B Polyclonal antibody

FAM131B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26498-1-AP

Synonyms

Protein FAM131B, KIAA0773

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using FAM131B Polyclonal antibody (26498-1-AP)

    WB analysis of PC-3 using 26498-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 26498-1-AP (FAM131B Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using FAM131B Polyclonal antibody (26498-1-AP)

    WB analysis of SH-SY5Y using 26498-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 26498-1-AP (FAM131B Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using FAM131B Polyclonal antibody (26498-1-AP)

    IHC staining of human brain using 26498-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26498-1-AP (FAM131B Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using FAM131B Polyclonal antibody (26498-1-AP)

    IHC staining of human brain using 26498-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26498-1-AP (FAM131B Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using FAM131B Polyclonal antibody (26498-1-AP)

    IF Staining of SH-SY5Y using 26498-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using FAM131B antibody (26498-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inPC-3 cells, SH-SY5Y cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26498-1-AP targets FAM131B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM131B fusion protein Ag24143 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 131, member B
Observed molecular weight 35-40 kDa
GenBank accession numberBC045611
Gene symbol FAM131B
Gene ID (NCBI) 9715
RRIDAB_2880534
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAM131B, also named as KIAA0773, has some isoforms with MW 35-40 kDa.

Protocols

Product Specific Protocols
WB protocol for FAM131B antibody 26498-1-APDownload protocol
IHC protocol for FAM131B antibody 26498-1-APDownload protocol
IF protocol for FAM131B antibody 26498-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols