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FAM134B Polyclonal antibody
FAM134B Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 21537-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | C2C12 cells, HEK-293 cells, rat brain tissue, mouse brain, mouse cerebellum |
| Positive IHC detected in | mouse brain tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 12 publications below |
| WB | See 36 publications below |
| IHC | See 1 publications below |
| IF | See 12 publications below |
| IP | See 1 publications below |
Product Information
21537-1-AP targets FAM134B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FAM134B fusion protein Ag16176 相同性解析による交差性が予測される生物種 |
| Full Name | family with sequence similarity 134, member B |
| Calculated molecular weight | 497 aa, 55 kDa |
| Observed molecular weight | 65 kDa |
| GenBank accession number | BC030517 |
| Gene symbol | FAM134B |
| Gene ID (NCBI) | 54463 |
| RRID | AB_2878879 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FAM134B, also known as the reticulophagy regulator 1 (RETREG1) or JK-1, is a member of the family with sequence similarity 134. FAM134B is an important ER-phagy receptor. FAM134B regulates the size and shape of the ER and participates in some ER-phagy-related processes(PMID: 33199694). FAM134B inhibition contributes to impair proteostasis in the ER due to the accumulation of misfolded or aggregated proteins, which in turn leads to compromised neuronal survival and progressive neuronal degenerative diseases. In addition, FAM134B mutations are common in patients with colorectal adenocarcinoma, and oesophageal squamous cell carcinoma(PMID: 29226326).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FAM134B antibody 21537-1-AP | Download protocol |
| IHC protocol for FAM134B antibody 21537-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Cells Deploy a Two-Pronged Strategy to Rectify Misfolded Proinsulin Aggregates.
| ||
Nat Commun Coordinate regulation of mutant NPC1 degradation by selective ER autophagy and MARCH6-dependent ERAD. | ||
Dev Cell Calnexin controls TrkB cell surface transport and ER-phagy in mouse cerebral cortex development | ||
Autophagy Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH H (Verified Customer) (04-06-2020) | The antibody works well. There were a few non-specific bands in HEK293T lysates though.
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