Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of HEK-293 using 21537-1-AP
WB result of FAM134B antibody (21537-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAM134B transfected HEK-293 cells.
WB analysis using 21537-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21537-1-AP (FAM134B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 21537-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21537-1-AP (FAM134B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 21537-1-AP
IP result of anti-FAM134B (IP:21537-1-AP, 4ug; Detection:21537-1-AP 1:5000) with Jurkat cells lysate 1600 ug.
IHC staining of mouse heart using 21537-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21537-1-AP (FAM134B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 21537-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21537-1-AP (FAM134B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 21537-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21537-1-AP (FAM134B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 21537-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21537-1-AP (FAM134B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

FAM134B Polyclonal antibody

FAM134B Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 21537-1-AP

Publications(46)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

RETREG1, Reticulophagy regulator 1, Reticulophagy receptor 1, JK1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (46)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	C2C12 cells, HEK-293 cells, rat brain tissue, mouse brain, mouse cerebellum
Positive IP detected in	Jurkat cells
Positive IHC detected in	mouse brain tissue, mouse heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 12 publications below
WB	See 40 publications below
IHC	See 1 publications below
IF	See 14 publications below
IP	See 1 publications below

Product Information

21537-1-AP targets FAM134B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FAM134B fusion protein Ag16176 相同性解析による交差性が予測される生物種
Full Name	family with sequence similarity 134, member B
Calculated molecular weight	497 aa, 55 kDa
Observed molecular weight	65 kDa
GenBank accession number	BC030517
Gene Symbol	FAM134B
Gene ID (NCBI)	54463
RRID	AB_2878879
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H6L5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FAM134B, also known as the reticulophagy regulator 1 (RETREG1) or JK-1, is a member of the family with sequence similarity 134. FAM134B is an important ER-phagy receptor. FAM134B regulates the size and shape of the ER and participates in some ER-phagy-related processes(PMID: 33199694). FAM134B inhibition contributes to impair proteostasis in the ER due to the accumulation of misfolded or aggregated proteins, which in turn leads to compromised neuronal survival and progressive neuronal degenerative diseases. In addition, FAM134B mutations are common in patients with colorectal adenocarcinoma, and oesophageal squamous cell carcinoma(PMID: 29226326).

Protocols

Product Specific Protocols
WB protocol for FAM134B antibody 21537-1-AP	Download protocol
IHC protocol for FAM134B antibody 21537-1-AP	Download protocol
IP protocol for FAM134B antibody 21537-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Cell Biol Combinatorial selective ER-phagy remodels the ER during neurogenesis Authors - Melissa J Hoyer View Article
human	WB,IF	Nat Commun AMFR-mediated Flavivirus NS2A ubiquitination subverts ER-phagy to augment viral pathogenicity Authors - Linliang Zhang View Article
human	WB	Mol Cell Cells Deploy a Two-Pronged Strategy to Rectify Misfolded Proinsulin Aggregates. Authors - Corey N Cunningham KD Validated View Article
human	WB	Nat Commun Coordinate regulation of mutant NPC1 degradation by selective ER autophagy and MARCH6-dependent ERAD. Authors - Mark L Schultz View Article
mouse	WB,IF	Dev Cell Calnexin controls TrkB cell surface transport and ER-phagy in mouse cerebral cortex development Authors - Patrick Lüningschrör View Article
human	WB	Autophagy Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy. Authors - Jonathan A Stefely View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 21537-1-AP

WB analysis using 21537-1-AP

WB analysis using 21537-1-AP

IP experiment of Jurkat using 21537-1-AP

IHC staining of mouse heart using 21537-1-AP

IHC staining of mouse brain using 21537-1-AP

IHC staining of mouse heart using 21537-1-AP

IHC staining of mouse brain using 21537-1-AP

FAM134B Polyclonal antibody

FAM134B Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Combinatorial selective ER-phagy remodels the ER during neurogenesis

AMFR-mediated Flavivirus NS2A ubiquitination subverts ER-phagy to augment viral pathogenicity

Cells Deploy a Two-Pronged Strategy to Rectify Misfolded Proinsulin Aggregates.

Coordinate regulation of mutant NPC1 degradation by selective ER autophagy and MARCH6-dependent ERAD.

Calnexin controls TrkB cell surface transport and ER-phagy in mouse cerebral cortex development

Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy.