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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAM135B Polyclonal antibody

FAM135B Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 28061-1-AP

Synonyms

C8ORFK32, FAM135B, Protein FAM135B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAM135B Polyclonal antibody (28061-1-AP)

    WB analysis using 28061-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28061-1-AP (FAM135B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using FAM135B Polyclonal antibody (28061-1-AP)

    IHC staining of human kidney using 28061-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28061-1-AP (FAM135B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using FAM135B Polyclonal antibody (28061-1-AP)

    IHC staining of human kidney using 28061-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28061-1-AP (FAM135B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FAM135B Polyclonal antibody (28061-1-AP)

    IHC staining of mouse brain using 28061-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28061-1-AP (FAM135B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FAM135B Polyclonal antibody (28061-1-AP)

    IHC staining of mouse brain using 28061-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28061-1-AP (FAM135B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using FAM135B Polyclonal antibody (28061-1-AP)

    IF Staining of mouse brain using 28061-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FAM135B antibody (28061-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using FAM135B Polyclonal antibody (28061-1-AP)

    IF Staining of mouse brain using 28061-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FAM135B antibody (28061-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inC6 cells, Neuro-2a cells
Positive IHC detected inhuman kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28061-1-AP targets FAM135B in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Rat, mouse samples.

Tested Reactivity Human, Rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM135B fusion protein Ag27897 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 135, member B
Observed molecular weight126-155 kDa
GenBank accession numberBC106912
Gene symbol FAM135B
Gene ID (NCBI) 51059
RRIDAB_2881050
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FAM135B antibody 28061-1-APDownload protocol
IHC protocol for FAM135B antibody 28061-1-APDownload protocol
IF protocol for FAM135B antibody 28061-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols