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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAM160B2 Polyclonal antibody

FAM160B2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17147-1-AP

Synonyms

Retinoic acid-induced protein 16, RAI16, FP13191, FHIP2B, FHF complex subunit HOOK-interacting protein 2B

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using FAM160B2 Polyclonal antibody (17147-1-AP)

    WB analysis of human brain using 17147-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17147-1-AP (FAM160B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using FAM160B2 Polyclonal antibody (17147-1-AP)

    WB analysis of human brain using 17147-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17147-1-AP (FAM160B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using FAM160B2 Polyclonal antibody (17147-1-AP)

    IP experiment of HepG2 using 17147-1-AP

    IP result of anti-FAM160B2 (IP:17147-1-AP, 4ug; Detection:17147-1-AP 1:300) with HepG2 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human brain tissue using FAM160B2 Polyclonal antibody (17147-1-AP)

    IHC staining of human brain using 17147-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17147-1-AP (FAM160B2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using FAM160B2 Polyclonal antibody (17147-1-AP)

    IHC staining of human brain using 17147-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17147-1-AP (FAM160B2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using FAM160B2 Polyclonal antibody (17147-1-AP)

    IF Staining of A549 using 17147-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 17147-1-AP (FAM160B2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inhuman brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17147-1-AP targets FAM160B2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM160B2 fusion protein Ag10920 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 160, member B2
Calculated molecular weight 743 aa, 82 kDa
Observed molecular weight 82 kDa
GenBank accession numberBC012865
Gene symbol FAM160B2
Gene ID (NCBI) 64760
RRIDAB_2246707
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAM160B2, also named as RAI16, may play a role in early lens development in mammals and in upregulation of genes in CD34+ cells in coronary artery disease patients.

Protocols

Product Specific Protocols
WB protocol for FAM160B2 antibody 17147-1-APDownload protocol
IHC protocol for FAM160B2 antibody 17147-1-APDownload protocol
IF protocol for FAM160B2 antibody 17147-1-APDownload protocol
IP protocol for FAM160B2 antibody 17147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell Endocrinol

Identification of retinoid acid induced 16 as a novel androgen receptor target in prostate cancer cells.

Authors - Cui-Ling Ding
View Article