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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAM162A Polyclonal antibody (32034-1-AP)

    WB analysis using 32034-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32034-1-AP (FAM162A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using FAM162A Polyclonal antibody (32034-1-AP)

    IF Staining of A431 using 32034-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using FAM162A antibody (32034-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

FAM162A Polyclonal antibody

FAM162A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32034-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Growth and transformation-dependent protein, FWP001, E2-induced gene 5 protein, E2IG5, DC16

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCaki-2 cells, HepG2 cells, RT-4 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32034-1-AP targets FAM162A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM162A fusion protein Ag36569 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 162, member A
Calculated molecular weight 154 aa, 17 kDa
Observed molecular weight17 kDa
GenBank accession numberBC015060
Gene Symbol FAM162A
Gene ID (NCBI) 26355
RRIDAB_3670175
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ96A26
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Family with sequence similarity 162 member A (FAM162A, also known as E2IG5, HGTD-P, and C3orf28) is a mitochondrial apoptotic protein, which is known as an HIF-1 alpha-responsive pro-apoptotic molecule (PMID: 15082785; 16698020; 22776087). When FAM162A was overexpressed, the hypoxia signal was sent directly to the mitochondria and caused cell death (PMID: 16698020).

Protocols

Product Specific Protocols
WB protocol for FAM162A antibody 32034-1-APDownload protocol
IF protocol for FAM162A antibody 32034-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols