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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAM185A Recombinant antibody

FAM185A Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240804F7

Cat no : 83921-3-RR

Synonyms

240804F7

Validation Data Gallery

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  • Western Blot (WB) analysis of HT-29 cells using FAM185A Recombinant antibody (83921-3-RR)

    WB analysis of HT-29 using 83921-3-RR

    HT-29 cells were subjected to SDS PAGE followed by western blot with 83921-3-RR (FAM185A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using FAM185A Recombinant antibody (83921-3-RR)

    IHC staining of human ovarian cancer using 83921-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83921-3-RR (FAM185A antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83921-3-RR

    Affinity and Kinetic Characterization of 83921-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83921-3-RR against Human FAM185A were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHT-29 cells
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83921-3-RR is FAM185A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FAM185A fusion protein Ag15041 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 185, member A
Calculated molecular weight 393 aa, 42 kDa
Observed molecular weight45 kDa
GenBank accession numberBC029175
Gene symbol FAM185A
Gene ID (NCBI) 222234
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FAM185A antibody 83921-3-RRDownload protocol
IHC protocol for FAM185A antibody 83921-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols