Validation Data Gallery
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Tested Applications
| Positive WB detected in | PC-3 cells |
| Positive IHC detected in | human prostate cancer tissue, human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
25752-1-AP targets COX20 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | COX20 fusion protein Ag22520 相同性解析による交差性が予測される生物種 |
| Full Name | family with sequence similarity 36, member A |
| Calculated molecular weight | 118 aa, 13 kDa |
| Observed molecular weight | ~16 kDa |
| GenBank accession number | BC018519 |
| Gene Symbol | COX20 |
| Gene ID (NCBI) | 116228 |
| RRID | AB_2880224 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5RI15 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
COX20, also name as FAM36A, belongs to the COX20 family. Mutations in COX20 are a novel cause of recessively inherited.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for COX20 antibody 25752-1-AP | Download protocol |
| IHC protocol for COX20 antibody 25752-1-AP | Download protocol |
| IF protocol for COX20 antibody 25752-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Ribosome ADP-ribosylation inhibits translation and maintains proteostasis in cancers. | ||
Sci Transl Med NIT2 dampens BRD1 phase separation and restrains oxidative phosphorylation to enhance chemosensitivity in gastric cancer | ||
Sci Data Bioinformatic analysis of membrane and associated proteins in murine cardiomyocytes and human myocardium. | ||
Front Endocrinol (Lausanne) Novel prognostic features and personalized treatment strategies for mitochondria-related genes in glioma patients |