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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Piezo1 (extracellular domain) Recombinant antibody (82625-4-RR)

    WB analysis using 82625-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82625-4-RR (Ag7791 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Piezo1 (extracellular domain) Recombinant antibody (82625-4-RR)

    WB analysis using 82625-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82625-4-RR (Piezo1 (extracellular domain) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat dorsal root ganglion tissue using Piezo1 (extracellular domain) Recombinant antibody (82625-4-RR)

    IHC staining of rat dorsal root ganglion using 82625-4-RR

    Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 82625-4-RR (Piezo1 (extracellular domain) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using Piezo1 (extracellular domain) Recombinant antibody (82625-4-RR)

    IF Staining of THP-1 using 82625-4-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using Piezo1 (extracellular domain) antibody (82625-4-RR, Clone: 4B15 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 82625-4-RR

    Affinity and Kinetic Characterization of 82625-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82625-4-RR against Human Piezo1 (extracellular domain) were performed. The affinity constant is below 1 pM.

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Piezo1 (extracellular domain) Recombinant antibody

Piezo1 (extracellular domain) Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 82625-4-RR
Clone No.4B15

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FAM38A, PIEZO1, 4B15, KIAA0233, Membrane protein induced by beta-amyloid treatment

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, SGC-7901 cells, hTERT-RPE1 cells
Positive IHC detected inrat dorsal root ganglion tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82625-4-RR targets Piezo1 (extracellular domain) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Piezo1 (extracellular domain) fusion protein Ag7791 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 38, member A
Calculated molecular weight 286 kDa
Observed molecular weight 233-286 kDa
GenBank accession numberBC008073
Gene Symbol Piezo1/FAM38A
Gene ID (NCBI) 9780
RRIDAB_3670524
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92508
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells that influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as Piezo1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Piezo1 also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in the gene encoding Piezo1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers. This antibody was raised against the extracellular domain of human Piezo1. (PMID: 20016066, 28416610)

Protocols

Product Specific Protocols
WB protocol for Piezo1 (extracellular domain) antibody 82625-4-RRDownload protocol
IHC protocol for Piezo1 (extracellular domain) antibody 82625-4-RRDownload protocol
IF protocol for Piezo1 (extracellular domain) antibody 82625-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols