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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAM3B Polyclonal antibody

FAM3B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27131-1-AP

Synonyms

C21orf11, C21orf76, Cytokine like protein 2 21, FAM3B, ORF9, Pancreatic derived factor, PANDER, PRED44, Protein FAM3B, UNQ320/PRO365

Validation Data Gallery

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  • Western Blot (WB) analysis of human milk using FAM3B Polyclonal antibody (27131-1-AP)

    WB analysis of human milk using 27131-1-AP

    human milk were subjected to SDS PAGE followed by western blot with 27131-1-AP (FAM3B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using FAM3B Polyclonal antibody (27131-1-AP)

    IHC staining of human pancreas using 27131-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 27131-1-AP (FAM3B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using FAM3B Polyclonal antibody (27131-1-AP)

    IHC staining of human pancreas using 27131-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 27131-1-AP (FAM3B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using FAM3B Polyclonal antibody (27131-1-AP)

    IHC staining of mouse pancreas using 27131-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 27131-1-AP (FAM3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using FAM3B Polyclonal antibody (27131-1-AP)

    IHC staining of mouse pancreas using 27131-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 27131-1-AP (FAM3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman milk tissue
Positive IHC detected inhuman pancreas tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 2 publications below

Product Information

The immunogen of 27131-1-AP is FAM3B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM3B fusion protein Ag25955 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 3, member B
Calculated molecular weight 26 kDa
Observed molecular weight 26 kDa, 23 kDa
GenBank accession numberBC057829
Gene symbol FAM3B
Gene ID (NCBI) 54097
RRIDAB_2880766
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAM3B, also known as Pancreatic-derived factor (PANDER), is a secreted glycoprotein that belongs to the cytokine like-protein family. There are four members in this gene family (FAM3A, FAM3B, FAM3C and FAM3D). As a result of alternative splicing and alternate signal peptide cleavage sites, multiple isoforms are known. FAM3B is selectively expressed at a high level in pancreatic islets and at low levels in the small intestine, prostate, and certain neurons.

Protocols

Product Specific Protocols
WB protocol for FAM3B antibody 27131-1-APDownload protocol
IHC protocol for FAM3B antibody 27131-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Int J Gen Med

FAM3B Serves as a Biomarker for the Development and Malignancy of Oral Lichen Planus.

Authors - Wenfang Wang
View Article
humanIHC

Front Pharmacol

A senescence-associated signature refines the classification of different modification patterns and characterization of tumor immune microenvironment infiltration in triple-negative breast cancer

Authors - Renhong Huang
View Article