FAM3C Polyclonal antibody

Name: FAM3C Polyclonal antibody
Brand: Proteintech
SKU: 14247-1-AP

FAM3C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14247-1-AP

Synonyms

FAM3C, GS3786, ILEI, Interleukin like EMT inducer, Protein FAM3C

Validation Data Gallery

Filter:

AllWBIHC

WB analysis using 14247-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14247-1-AP (FAM3C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 14247-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 14247-1-AP (FAM3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 14247-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 14247-1-AP (FAM3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14247-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14247-1-AP (FAM3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 14247-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14247-1-AP (FAM3C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas using 14247-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 14247-1-AP (FAM3C antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 14247-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 14247-1-AP (FAM3C antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human stomach cancer using 14247-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14247-1-AP (FAM3C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 14247-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14247-1-AP (FAM3C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 14247-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 14247-1-AP (FAM3C antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human stomach cancer using 14247-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 14247-1-AP (FAM3C antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 14247-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HCT 116 cells, human brain tissue, human heart tissue, HEK-293 cells, HT-29 cells, HepG2 cells
Positive IHC detected in	human pancreas tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IHC	See 3 publications below

Product Information

14247-1-AP targets FAM3C in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FAM3C fusion protein Ag5516 相同性解析による交差性が予測される生物種
Full Name	family with sequence similarity 3, member C
Calculated molecular weight	25 kDa
Observed molecular weight	25 kDa
GenBank accession number	BC046932
Gene symbol	FAM3C
Gene ID (NCBI)	10447
RRID	AB_2278149
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FAM3C, also known as ILEI, is a secreted protein belonging to the cytokine-like protein family. FAM3C is a signaling cytokine with a GG domain, hydrophobic leader sequence and an N-terminal signal peptide. A change in expression of this protein has been noted in pancreatic cancer-derived cells. FAM3C has been involved in the epithelial to mesenchymal transition, and retinal laminar formation processes in vertebrates. Also, FAM3C may play an important role in inner ear development and thus be involved in cell differentiation and proliferation during inner ear embryogenesis.

Protocols

Product Specific Protocols
WB protocol for FAM3C antibody 14247-1-AP	Download protocol
IHC protocol for FAM3C antibody 14247-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Sci Adv p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi. Authors - Xiaochao Tan View Article
human	WB	J Proteome Res Mining the gastric cancer secretome: identification of GRN as a potential diagnostic marker for early gastric cancer. Authors - Loei Hendrick H View Article
human	WB	Diagn Pathol Prognostic significance of FAM3C in esophageal squamous cell carcinoma. Authors - Ying-Hui Zhu View Article
human	IHC	Int J Clin Exp Pathol Overexpression of FAM3C protein as a novel biomarker for epithelial-mesenchymal transition and poor outcome in gastric cancer. Authors - Shuai Yin View Article
human,mouse	WB,IHC	Int J Biol Sci A Novel ER Stress Mediator TMTC3 Promotes Squamous Cell Carcinoma Progression by Activating GRP78/PERK Signaling Pathway Authors - Hongyu Yuan View Article
human	IHC	Sci Rep FAM3 family genes are associated with prognostic value of human cancer: a pan-cancer analysis Authors - Qing-Tai Dong View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAM3C Polyclonal antibody

FAM3C Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 14247-1-AP

WB analysis of human brain using 14247-1-AP

WB analysis of human heart using 14247-1-AP

WB analysis of HEK-293 using 14247-1-AP

WB analysis using 14247-1-AP

IHC staining of human pancreas using 14247-1-AP

IHC staining of human pancreas using 14247-1-AP

IHC staining of human stomach cancer using 14247-1-AP

IHC staining of human stomach cancer using 14247-1-AP

IHC staining of human stomach cancer using 14247-1-AP

IHC staining of human stomach cancer using 14247-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.

Mining the gastric cancer secretome: identification of GRN as a potential diagnostic marker for early gastric cancer.

Prognostic significance of FAM3C in esophageal squamous cell carcinoma.

Overexpression of FAM3C protein as a novel biomarker for epithelial-mesenchymal transition and poor outcome in gastric cancer.

A Novel ER Stress Mediator TMTC3 Promotes Squamous Cell Carcinoma Progression by Activating GRP78/PERK Signaling Pathway

FAM3 family genes are associated with prognostic value of human cancer: a pan-cancer analysis