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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAM3D Polyclonal antibody

FAM3D Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12336-1-AP

Synonyms

UNQ567/PRO1130, Protein FAM3D, OIT1, EF7

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse colon tissue using FAM3D Polyclonal antibody (12336-1-AP)

    WB analysis of mouse colon using 12336-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 12336-1-AP (FAM3D antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat colon tissue using FAM3D Polyclonal antibody (12336-1-AP)

    WB analysis of rat colon using 12336-1-AP

    rat colon tissue were subjected to SDS PAGE followed by western blot with 12336-1-AP (FAM3D antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using FAM3D Polyclonal antibody (12336-1-AP)

    IHC staining of human stomach cancer using 12336-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12336-1-AP (FAM3D antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse colon tissue, rat colon tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12336-1-AP targets FAM3D in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM3D fusion protein Ag2965 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 3, member D
Calculated molecular weight 224 aa, 24 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC015359
Gene symbol FAM3D
Gene ID (NCBI) 131177
RRIDAB_10667402
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAM3 (family with sequence similarity 3) is a cytokine-like family consisting of four members, FAM3A, FAM3B, FAM3C, and FAM3D, who share a 4-helical bundle structure. FAM3D is constitutively expressed in the gastrointestinal tract and is regulated by nutritional status. FAM3D controls blood sugar levels by acting as an insulin-sensitizing protein and improves lipid deposition in the liver. (PMID: 22226334; PMID: 37328068)

Protocols

Product Specific Protocols
WB protocol for FAM3D antibody 12336-1-APDownload protocol
IHC protocol for FAM3D antibody 12336-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

DNA methylation-driven gene FAM3D promotes colorectal cancer growth via the ATF4-SESN2-mTORC1 pathway

Authors - Ting Zheng
  • KO Validated
View Article