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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using FAM48A Polyclonal antibody (24419-1-AP)

    WB analysis of PC-3 using 24419-1-AP (same clone as 24419-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 24419-1-AP (FAM48A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24419-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using FAM48A Polyclonal antibody (24419-1-AP)

    WB analysis of Jurkat using 24419-1-AP (same clone as 24419-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24419-1-AP (FAM48A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24419-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using FAM48A Polyclonal antibody (24419-1-AP)

    WB analysis of PC-3 using 24419-1-AP (same clone as 24419-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 24419-1-AP (FAM48A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24419-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FAM48A Polyclonal antibody (24419-1-AP)

    IHC staining of mouse testis using 24419-1-AP (same clone as 24419-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24419-1-AP (FAM48A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24419-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FAM48A Polyclonal antibody (24419-1-AP)

    IHC staining of mouse testis using 24419-1-AP (same clone as 24419-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24419-1-AP (FAM48A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24419-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using FAM48A Polyclonal antibody (24419-1-AP)

    IHC staining of human testis using 24419-1-AP (same clone as 24419-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24419-1-AP (FAM48A Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24419-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using FAM48A Polyclonal antibody (24419-1-AP)

    IHC staining of human testis using 24419-1-AP (same clone as 24419-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24419-1-AP (FAM48A Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24419-1-PBS in a different storage buffer formulation.

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FAM48A Polyclonal antibody, PBS Only

FAM48A Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24419-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24419-1-PBS targets FAM48A in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM48A fusion protein Ag19875 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 48, member A
Calculated molecular weight 779 aa, 86 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC030686
Gene Symbol FAM48A
Gene ID (NCBI) 55578
RRIDAB_2879537
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NEM7
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

FAM48A also named as C13orf19 or SUPT20H is a 779 amino acid protein, which belongs to the SPT20 family. FAM48A is required for MAP kinase p38 activation during gastrulation and for starvation-induced ATG9A trafficking during autophagy. FAM48A localizes in the nucleus and is highly expressed in testis, moderately in brain and pituitary gland.