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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

ESYT2 Polyclonal antibody

ESYT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24385-1-AP

Synonyms

FAM62B, Extended synaptotagmin-2, Extended synaptotagmin 2, E-Syt2, E Syt2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAM62B Polyclonal antibody (24385-1-AP)

    WB analysis using 24385-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24385-1-AP (FAM62B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FAM62B Polyclonal antibody (24385-1-AP)

    WB analysis of HeLa using 24385-1-AP

    WB result of FAM62B antibody (24385-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAM62B transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using ESYT2 Polyclonal antibody (24385-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 24385-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 24385-1-AP (ESYT2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using ESYT2 Polyclonal antibody (24385-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 24385-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 24385-1-AP (ESYT2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inK-562 cells, HeLa cells, LNCaP cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24385-1-AP targets ESYT2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ESYT2 fusion protein Ag20420 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 62 (C2 domain containing) member B
Calculated molecular weight 921 aa, 102 kDa
Observed molecular weight90-100 kDa
GenBank accession numberBC013957
Gene symbol ESYT2
Gene ID (NCBI) 57488
RRIDAB_2879519
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ESYT2, also named FAM62B, one of the three-member family of Extended Synaptotagmins that were recently shown to be implicated in the formation of endoplasmic reticulum (ER)-plasma membrane (PM) junctions and in the Ca(2+) dependent regulation of these junctions.

Protocols

Product Specific Protocols
WB protocol for ESYT2 antibody 24385-1-APDownload protocol
IHC protocol for ESYT2 antibody 24385-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

The resealing factor S100A11 interacts with annexins and extended synaptotagmin-1 in the course of plasma membrane wound repair

Authors - Arsila P. K. Ashraf
  • KD Validated
View Article