Validation Data Gallery
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Tested Applications
| Positive WB detected in | K-562 cells, HeLa cells, LNCaP cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
Product Information
24385-1-AP targets ESYT2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ESYT2 fusion protein Ag20420 相同性解析による交差性が予測される生物種 |
| Full Name | family with sequence similarity 62 (C2 domain containing) member B |
| Calculated molecular weight | 921 aa, 102 kDa |
| Observed molecular weight | 90-100 kDa |
| GenBank accession number | BC013957 |
| Gene Symbol | ESYT2 |
| Gene ID (NCBI) | 57488 |
| RRID | AB_2879519 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A0FGR8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ESYT2, also named FAM62B, one of the three-member family of Extended Synaptotagmins that were recently shown to be implicated in the formation of endoplasmic reticulum (ER)-plasma membrane (PM) junctions and in the Ca(2+) dependent regulation of these junctions.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ESYT2 antibody 24385-1-AP | Download protocol |
| IHC protocol for ESYT2 antibody 24385-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol The resealing factor S100A11 interacts with annexins and extended synaptotagmin-1 in the course of plasma membrane wound repair
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