Validation Data Gallery
Tested Applications
| Positive WB detected in | human skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
17015-1-AP targets FAM65B in WB, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FAM65B fusion protein Ag9991 相同性解析による交差性が予測される生物種 |
| Full Name | family with sequence similarity 65, member B |
| Calculated molecular weight | 118 kDa |
| Observed molecular weight | 115 kDa, 66 kDa |
| GenBank accession number | BC001232 |
| Gene Symbol | FAM65B |
| Gene ID (NCBI) | 9750 |
| RRID | AB_10640810 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y4F9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FAM65B is also named as C6orf32, DIFF48, KIAA0386, PL48 and belongs to the FAM65 family.It has 2 isoforms produced by alternative splicing.Isoform 2 play a role in promoting myogenic cell differentiation, cytoskeletal rearrangement and filopodia formation(PMID:17150207). FAM65B is expressed in mononuclear cells, not in myotubes, upon induction of differentiation and cellular fusion, and expression is upregulated during human myoblast differentiation at both the mRNA and protein levels(PMID:17150207).This antibody is specific to the isoform of 119 kDa of FAM65B.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FAM65B antibody 17015-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A FAM65B is a membrane-associated protein of hair cell stereocilia required for hearing. | ||
J Cell Sci Front Signal-Dependent Accumulation of RHOA Inhibitor FAM65B at Leading Edges Polarizes Neutrophils.
| ||
FASEB J Fam65b is important for formation of the HDAC6-dysferlin protein complex during myogenic cell differentiation. |