Validation Data Gallery
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Tested Applications
| Positive WB detected in | HT-29 cells, mouse spleen tissue, HeLa cells, Raji cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
21203-1-AP targets FAM72 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FAM72 fusion protein Ag15566 相同性解析による交差性が予測される生物種 |
| Full Name | family with sequence similarity 72, member A |
| Calculated molecular weight | 149 aa, 17 kDa |
| Observed molecular weight | 15-20 kDa |
| GenBank accession number | BC035696 |
| Gene Symbol | FAM72 |
| Gene ID (NCBI) | 729533 |
| RRID | AB_10733346 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5TYM5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FAM72A, also named as LMIP and UGENE, is a mitochondrial protein which regulates cellular reactive oxygen species metabolism. It's up-regulated in malignant colon cancers, compared to normal colon and colon adenomas. FAM72 has some members A/B/C/D with higher homology. This antibody detects all the members of FAM72.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FAM72 antibody 21203-1-AP | Download protocol |
| IHC protocol for FAM72 antibody 21203-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Oncol Integrated systemic analysis of FAM72A to identify its clinical relevance, biological function, and relationship to drug sensitivity in hepatocellular carcinoma
| ||
Int J Mol Sci Prognostic and Immunological Implications of FAM72A in Pan-Cancer and Functional Validations
|