Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, U2O2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29811-1-AP targets MIGA2/FAM73B in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MIGA2/FAM73B fusion protein Ag30577 相同性解析による交差性が予測される生物種 |
| Full Name | family with sequence similarity 73, member B |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | BC009114 |
| Gene Symbol | MIGA2/FAM73B |
| Gene ID (NCBI) | 84895 |
| RRID | AB_3086170 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7L4E1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MIGA2 (mitoguardin-2) also named as FAM73B, is a mitochondrial protein at contacts with the ER and/or lipid droplets (LDs), and identified as a lipid transporter. MIGA2 localizes to contact sites between mitochondria and the ER or mitochondria and lipid droplets (LDs) (PMID: 36282247). Based on its presence in many tissues, MIGA2 is likely critical for lipid and energy homeostasis in a wide spectrum of cell types (PMID: 31628041).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MIGA2/FAM73B antibody 29811-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |