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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAM78A Polyclonal antibody

FAM78A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21038-1-AP

Synonyms

Protein FAM78A, C9orf59

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using FAM78A Polyclonal antibody (21038-1-AP)

    WB analysis of mouse liver using 21038-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 21038-1-AP (FAM78A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using FAM78A Polyclonal antibody (21038-1-AP)

    WB analysis of mouse spleen using 21038-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21038-1-AP (FAM78A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using FAM78A Polyclonal antibody (21038-1-AP)

    WB analysis of human liver using 21038-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 21038-1-AP (FAM78A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using FAM78A Polyclonal antibody (21038-1-AP)

    WB analysis of HepG2 using 21038-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21038-1-AP (FAM78A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using FAM78A Polyclonal antibody (21038-1-AP)

    WB analysis of mouse spleen using 21038-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21038-1-AP (FAM78A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using FAM78A Polyclonal antibody (21038-1-AP)

    IHC staining of human tonsillitis using 21038-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 21038-1-AP (FAM78A Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using FAM78A Polyclonal antibody (21038-1-AP)

    IHC staining of human tonsillitis using 21038-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 21038-1-AP (FAM78A Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FAM78A Polyclonal antibody (21038-1-AP)

    IHC staining of human colon cancer using 21038-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 21038-1-AP (FAM78A Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FAM78A Polyclonal antibody (21038-1-AP)

    IHC staining of human colon cancer using 21038-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 21038-1-AP (FAM78A Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FAM78A Polyclonal antibody (21038-1-AP)

    IF Staining of HepG2 using 21038-1-AP

    Immunofluorescent analysis of HepG2 cells using 21038-1-AP (FAM78A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse liver tissue, HepG2 cells, human liver tissue, mouse spleen tissue
Positive IHC detected inhuman tonsillitis tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21038-1-AP targets FAM78A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM78A fusion protein Ag15366 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 78, member A
Calculated molecular weight 283 aa, 32 kDa
Observed molecular weight 32-35 kDa
GenBank accession numberBC049388
Gene symbol FAM78A
Gene ID (NCBI) 286336
RRIDAB_10973677
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FAM78A antibody 21038-1-APDownload protocol
IHC protocol for FAM78A antibody 21038-1-APDownload protocol
IF protocol for FAM78A antibody 21038-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols