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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAM83G Polyclonal antibody (29366-1-AP)

    WB analysis using 29366-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29366-1-AP (FAM83G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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FAM83G Polyclonal antibody

FAM83G Polyclonal Antibody for WB, ELISA
Cat No. 29366-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Protein FAM83G

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inBxPC-3 cells, HCT 116 cells, HepG2 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29366-1-AP targets FAM83G in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM83G fusion protein Ag28925 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 83, member G
Calculated molecular weight 91 kDa
Observed molecular weight100 kDa
GenBank accession numberNM_001039999
Gene Symbol FAM83G
Gene ID (NCBI) 644815
RRIDAB_3669686
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA6ND36
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAM83G is a member of the FAM83 protein family, which consists of eight members characterized by sharing a conserved N-terminal domain named DUF1669. This domain is homologous to the phospholipase D catalytic domain but lacks essential catalytic histidine residues, suggesting it is unlikely to have phospholipase activity. FAM83G, in particular, has a consensus motif for serine/threonine protein kinase D1/protein kinase C mu (PKD1/PKCμ) phosphorylation at serine residue 356 (S356), which suggests it may have a unique physiological function among the FAM83 protein family members. Furthermore, FAM83G has been identified as a novel inducer of apoptosis, with its phosphorylation at S356 modulating heat shock protein 27 (HSP27) phosphorylation and apoptosis regulation.

Protocols

Product Specific Protocols
WB protocol for FAM83G antibody 29366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols