Validation Data Gallery
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Tested Applications
| Positive WB detected in | MDA-MB-453s cells, Jurkat cells |
| Positive IP detected in | HUVEC cells |
| Positive IHC detected in | human liver cancer tissue, human heart tissue, human kidney tissue, human liver tissue, human lung tissue, human oesophagus cancer tissue, human ovary tissue, human skin tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 8 publications below |
| IHC | See 10 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
18421-1-AP targets FAM84B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FAM84B fusion protein Ag13243 相同性解析による交差性が予測される生物種 |
| Full Name | family with sequence similarity 84, member B |
| Calculated molecular weight | 34 kDa |
| Observed molecular weight | 40 kDa |
| GenBank accession number | BC052957 |
| Gene Symbol | FAM84B |
| Gene ID (NCBI) | 157638 |
| RRID | AB_2278161 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96KN1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FAM84B antibody 18421-1-AP | Download protocol |
| IHC protocol for FAM84B antibody 18421-1-AP | Download protocol |
| IP protocol for FAM84B antibody 18421-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Genet Genome-wide profiling of HPV integration in cervical cancer identifies clustered genomic hot spots and a potential microhomology-mediated integration mechanism. | ||
Gigascience Genomic analyses reveal FAM84B and the NOTCH pathway are associated with the progression of esophageal squamous cell carcinoma.
| ||
Proc Natl Acad Sci U S A An in vitro vesicle formation assay reveals cargo clients and factors that mediate vesicular trafficking.
| ||
Aging (Albany NY) FAM84B, amplified in pancreatic ductal adenocarcinoma, promotes tumorigenesis through the Wnt/β-catenin pathway. | ||