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KD/KO Validated

FAM84B Polyclonal antibody

Name: FAM84B Polyclonal antibody
Brand: Proteintech
SKU: 18421-1-AP

FAM84B Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 18421-1-AP

Synonyms

FAM84B, NSE2, Protein FAM84B, Protein NSE2

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis of MDA-MB-453s using 18421-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 18421-1-AP (FAM84B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 18421-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 18421-1-AP (FAM84B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of HUVEC using 18421-1-AP
IP result of anti-FAM84B (IP:18421-1-AP, 4ug; Detection:18421-1-AP 1:300) with HUVEC cells lysate 3600ug.
IHC staining of human liver cancer using 18421-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18421-1-AP (FAM84B Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver cancer using 18421-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18421-1-AP (FAM84B Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human ovary using 18421-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary using 18421-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human oesophagus cancer using 18421-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human oesophagus cancer using 18421-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 18421-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 18421-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 18421-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 18421-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 18421-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 18421-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 18421-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 18421-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin using 18421-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin using 18421-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human spleen using 18421-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human spleen using 18421-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung using 18421-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung using 18421-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 18421-1-AP (FAM84B antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 18421-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	MDA-MB-453s cells, Jurkat cells
Positive IP detected in	HUVEC cells
Positive IHC detected in	human liver cancer tissue, human heart tissue, human kidney tissue, human liver tissue, human lung tissue, human oesophagus cancer tissue, human ovary tissue, human skin tissue, human spleen tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 8 publications below
IHC	See 10 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

18421-1-AP targets FAM84B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FAM84B fusion protein Ag13243 相同性解析による交差性が予測される生物種
Full Name	family with sequence similarity 84, member B
Calculated molecular weight	34 kDa
Observed molecular weight	40 kDa
GenBank accession number	BC052957
Gene symbol	FAM84B
Gene ID (NCBI)	157638
RRID	AB_2278161
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for FAM84B antibody 18421-1-AP	Download protocol
IHC protocol for FAM84B antibody 18421-1-AP	Download protocol
IP protocol for FAM84B antibody 18421-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Nat Genet Genome-wide profiling of HPV integration in cervical cancer identifies clustered genomic hot spots and a potential microhomology-mediated integration mechanism. Authors - Zheng Hu View Article
human	WB,IHC	Gigascience Genomic analyses reveal FAM84B and the NOTCH pathway are associated with the progression of esophageal squamous cell carcinoma. Authors - Caixia Cheng KD Validated View Article
human	IF	Proc Natl Acad Sci U S A An in vitro vesicle formation assay reveals cargo clients and factors that mediate vesicular trafficking. Authors - Yan Huang KD Validated View Article
human	WB,IHC	Ther Adv Med Oncol FAM84B promotes prostate tumorigenesis through a network alteration. Authors - Yanzhi Jiang View Article
human	IHC	Aging (Albany NY) FAM84B, amplified in pancreatic ductal adenocarcinoma, promotes tumorigenesis through the Wnt/β-catenin pathway. Authors - Xin Zhang View Article
human,mouse	WB,IHC	Oncotarget Upregulation of FAM84B during prostate cancer progression. Authors - Nicholas Wong View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAM84B Polyclonal antibody

FAM84B Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of MDA-MB-453s using 18421-1-AP

WB analysis of Jurkat using 18421-1-AP

IP experiment of HUVEC using 18421-1-AP

IHC staining of human liver cancer using 18421-1-AP

IHC staining of human liver cancer using 18421-1-AP

IHC staining of human ovary using 18421-1-AP

IHC staining of human ovary using 18421-1-AP

IHC staining of human oesophagus cancer using 18421-1-AP

IHC staining of human oesophagus cancer using 18421-1-AP

IHC staining of human liver using 18421-1-AP

IHC staining of human liver using 18421-1-AP

IHC staining of human heart using 18421-1-AP

IHC staining of human heart using 18421-1-AP

IHC staining of human kidney using 18421-1-AP

IHC staining of human kidney using 18421-1-AP

IHC staining of human testis using 18421-1-AP

IHC staining of human testis using 18421-1-AP

IHC staining of human skin using 18421-1-AP

IHC staining of human skin using 18421-1-AP

IHC staining of human spleen using 18421-1-AP

IHC staining of human spleen using 18421-1-AP

IHC staining of human lung using 18421-1-AP

IHC staining of human lung using 18421-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Genome-wide profiling of HPV integration in cervical cancer identifies clustered genomic hot spots and a potential microhomology-mediated integration mechanism.

Genomic analyses reveal FAM84B and the NOTCH pathway are associated with the progression of esophageal squamous cell carcinoma.

An in vitro vesicle formation assay reveals cargo clients and factors that mediate vesicular trafficking.

FAM84B promotes prostate tumorigenesis through a network alteration.

FAM84B, amplified in pancreatic ductal adenocarcinoma, promotes tumorigenesis through the Wnt/β-catenin pathway.

Upregulation of FAM84B during prostate cancer progression.