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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAM92B/CIBAR2 Polyclonal antibody

FAM92B/CIBAR2 Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 26413-1-AP

Synonyms

FAM92B, Protein FAM92B, CIBAR2, CBY1-interacting BAR domain-containing protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FAM92B Polyclonal antibody (26413-1-AP)

    WB analysis of HeLa using 26413-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26413-1-AP (FAM92B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using FAM92B Polyclonal antibody (26413-1-AP)

    WB analysis of COLO 320 using 26413-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 26413-1-AP (FAM92B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using FAM92B Polyclonal antibody (26413-1-AP)

    WB analysis of mouse small intestine using 26413-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 26413-1-AP (FAM92B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse lung tissue using FAM92B/CIBAR2 Polyclonal antibody (26413-1-AP)

    IF Staining of mouse lung using 26413-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using FAM92B antibody (26413-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse lung tissue using FAM92B/CIBAR2 Polyclonal antibody (26413-1-AP)

    IF Staining of mouse lung using 26413-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using FAM92B antibody (26413-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, COLO 320 cells, mouse small intestine tissue
Positive IF-P detected inmouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

26413-1-AP targets FAM92B/CIBAR2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM92B/CIBAR2 fusion protein Ag24802 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 92, member B
Calculated molecular weight 304 aa, 35 kDa
Observed molecular weight 32-35 kDa
GenBank accession numberBC111944
Gene symbol FAM92B
Gene ID (NCBI) 339145
RRIDAB_2880506
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAM92B (family with sequence similarity 92, member B, also named CIBAR2) is a transmembrane protein that plays a crucial role in various cellular processes, including membrane trafficking and cell adhesion. The protein is predicted to be intracellular and is involved in ciliogenesis, likely through the recruitment and fusion of endosomal vesicles at distal appendages during early stages of ciliogenesis.

Protocols

Product Specific Protocols
WB protocol for FAM92B/CIBAR2 antibody 26413-1-APDownload protocol
IF protocol for FAM92B/CIBAR2 antibody 26413-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Life Sci Alliance

ciBAR1 loss in mice causes laterality defects, pancreatic degeneration, and altered glucose tolerance

Authors - Eunice N Kim
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Eunice (Verified Customer) (10-24-2022)

This was tested on mouse tracheal epithelial cells fixed in 4% PFA overnight and on MEFs fixed for 10 min in ice-cold methanol acetone. Works well.

  • Applications: Immunofluorescence, Cell culture
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Tracheal epithelial cells, mouse embryonic fibroblasts (MEFs)