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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAM98B Polyclonal antibody

FAM98B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22251-1-AP

Synonyms

Protein FAM98B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAM98B Polyclonal antibody (22251-1-AP)

    WB analysis using 22251-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22251-1-AP (FAM98B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FAM98B Polyclonal antibody (22251-1-AP)

    WB analysis using 22251-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22251-1-AP (FAM98B antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FAM98B Polyclonal antibody (22251-1-AP)

    IHC staining of human breast cancer using 22251-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22251-1-AP (FAM98B Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FAM98B Polyclonal antibody (22251-1-AP)

    IHC staining of human breast cancer using 22251-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22251-1-AP (FAM98B Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FAM98B Polyclonal antibody (22251-1-AP)

    IF Staining of HepG2 using 22251-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FAM98B antibody (22251-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHepG2 cells, Caco-2 cells, Raji cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22251-1-AP targets FAM98B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM98B fusion protein Ag17747 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 98, member B
Calculated molecular weight 330 aa, 37 kDa
Observed molecular weight 45-48 kDa
GenBank accession numberBC093898
Gene symbol FAM98B
Gene ID (NCBI) 283742
RRIDAB_2879049
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FAM98B antibody 22251-1-APDownload protocol
IHC protocol for FAM98B antibody 22251-1-APDownload protocol
IF protocol for FAM98B antibody 22251-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols