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KD/KO Validated

FANCA Polyclonal antibody

Name: FANCA Polyclonal antibody
Brand: Proteintech
SKU: 11975-1-AP

FANCA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11975-1-AP

Synonyms

FANCH, FAH, FACA, FAA, FA1

Validation Data Gallery

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AllWBIHC

WB analysis of HeLa cells using 11975-1-AP
WB results of FANCA (11975-1-AP, 1:500) with si-control and si-FANCA transfected HeLa cells.
WB analysis using 11975-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11975-1-AP (FANCA antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 11975-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11975-1-AP (FANCA Antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human colon cancer using 11975-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11975-1-AP (FANCA Antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human stomach cancer using 11975-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11975-1-AP (FANCA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 11975-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11975-1-AP (FANCA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse intestine using 11975-1-AP
Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 11975-1-AP (FANCA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse thymus using 11975-1-AP
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 11975-1-AP (FANCA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat small intestine using 11975-1-AP
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 11975-1-AP (FANCA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat testis using 11975-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11975-1-AP (FANCA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 11975-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	COLO 320 cells, HeLa cells
Positive IHC detected in	human colon cancer tissue, human cervical cancer tissue, human stomach cancer tissue, mouse intestine, mouse thymus tissue, rat small intestine tissue, rat testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 7 publications below
IHC	See 3 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

11975-1-AP targets FANCA in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FANCA fusion protein Ag2598 相同性解析による交差性が予測される生物種
Full Name	Fanconi anemia, complementation group A
Calculated molecular weight	163 kDa
Observed molecular weight	163 kDa
GenBank accession number	BC008979
Gene symbol	FANCA
Gene ID (NCBI)	2175
RRID	AB_2101786
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FANCA, also named as FAA, FACA and FANCH, is a DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. It's involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.

Protocols

Product Specific Protocols
WB protocol for FANCA antibody 11975-1-AP	Download protocol
IHC protocol for FANCA antibody 11975-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Nat Struct Mol Biol Fanconi anemia proteins participate in a break-induced-replication-like pathway to counter replication stress. Authors - Xinlin Xu KO Validated View Article
mouse	WB	EMBO Mol Med WNT inhibition creates a BRCA-like state in Wnt-addicted cancer. Authors - Amanpreet Kaur View Article
human	WB	Cell Rep C1orf112 teams up with FIGNL1 to facilitate RAD51 filament disassembly and DNA interstrand cross-link repair Authors - Zenan Zhou View Article
human	WB,IHC	Acta Biochim Biophys Sin (Shanghai) FANCA facilitates G1/S cell cycle advancement, proliferation, migration and invasion in gastric cancer Authors - Wei Wang KD Validated View Article
human	WB	Gene Circular RNA Circ_0000119 promotes gastric cancer progression via circ_0000119/miR-502-5p/MTBP axis Authors - Mengyan Xie View Article
human	IHC,IF,IP	Gene Identification of rare heterozygous missense mutations in FANCA in esophageal atresia patients using next-generation sequencing. Authors - Yu Feng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FANCA Polyclonal antibody

FANCA Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of HeLa cells using 11975-1-AP

WB analysis using 11975-1-AP

IHC staining of human colon cancer using 11975-1-AP

IHC staining of human colon cancer using 11975-1-AP

IHC staining of human stomach cancer using 11975-1-AP

IHC staining of human cervical cancer using 11975-1-AP

IHC staining of mouse intestine using 11975-1-AP

IHC staining of mouse thymus using 11975-1-AP

IHC staining of rat small intestine using 11975-1-AP

IHC staining of rat testis using 11975-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Fanconi anemia proteins participate in a break-induced-replication-like pathway to counter replication stress.

WNT inhibition creates a BRCA-like state in Wnt-addicted cancer.

C1orf112 teams up with FIGNL1 to facilitate RAD51 filament disassembly and DNA interstrand cross-link repair

FANCA facilitates G1/S cell cycle advancement, proliferation, migration and invasion in gastric cancer

Circular RNA Circ_0000119 promotes gastric cancer progression via circ_0000119/miR-502-5p/MTBP axis

Identification of rare heterozygous missense mutations in FANCA in esophageal atresia patients using next-generation sequencing.