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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FANCA-Specific Polyclonal antibody (19542-1-AP)

    WB analysis of HeLa using 19542-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19542-1-AP (FANCA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FANCA-Specific Polyclonal antibody (19542-1-AP)

    WB analysis of HeLa using 19542-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19542-1-AP (FANCA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using FANCA-Specific Polyclonal antibody (19542-1-AP)

    WB analysis of HEK-293 using 19542-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19542-1-AP (FANCA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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FANCA-Specific Polyclonal antibody

FANCA-Specific Polyclonal Antibody for WB, ELISA
Cat No. 19542-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

FA, FA H, FA1, FAA, FACA, FAH, FANCA, FANCA-Specific, FANCH, Fanconi anemia group A protein, Protein FACA

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19542-1-AP targets FANCA-Specific in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name Fanconi anemia, complementation group A
Calculated molecular weight 163 kDa
Observed molecular weight 163 kDa
GenBank accession numberNM_000135
Gene Symbol FANCA
Gene ID (NCBI) 2175
RRIDAB_10638755
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15360
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FANCA, also named as FAA, FACA and FANCH, is DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. FANCA may be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Defects in FANCA are a cause of Fanconi anemia (FA) which is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. The antibody is specific to isoform1 of FANCA.

Protocols

Product Specific Protocols
WB protocol for FANCA-Specific antibody 19542-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols